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- Abstract：Folic acid (FA) plays an important role in cell metabolism. In this study, we primarily looked at the protective effect of FA on PC12 cytotoxicity induced by lead acetate. PC12 cells were exposed to lead-free medium and 10μmol/L lead-acetate culture solution for 24h. Medium with FA proportions of 0mg/L, 0.5mg/L, 5mg/L, and 50mg/L continued to function. After 24h, 48h, and 72h, we collected PC12 cells, and detected cell proliferation or inhibition with the MTT method. We extracted cell protein and detected the activities of SOD, MDA, and GSH-PX in each group. The immunofluorescence method was used to study the expression of caspase-3 protein. Changes of Aβ40 and Aβ42 in the cell-culture fluid were detected with ELISA. The results showed that lead exposure inhibited cell growth (P < 0.05), increased intracellular MDA content, SOD activity, and caspase-3 protein expression (P < 0.05), and decreased GSH-PX activity and Aβ40 protein expression (P < 0.05). FA intervention can increase cell proliferation, enhance the activity of SOD and GSH-PX (P < 0.05), and reduce cell MDA content and caspase-3 protein expression (P < 0.05). There was no statistically significant difference between Aβ40 and Aβ42 content between the groups (P > 0.05). The study found that FA could be a highly effective approach against lead-associated PC12 cell toxicity.Keywords：Lead;Folic acid;PC12 cells;Oxidative stress;Apoptosis
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Updated：2022-06-07
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