利用<italic style="font-style: italic">Bacillus paramycoides</italic>细胞内蛋白延伸因子Tu揭示硒纳米颗粒的创新绿色合成机制

刘培; 龙海钰; 贺帅; 程翰; 李迩东; 成思宇; 梁梦娣; 刘正伟; 郭振; 时号

doi:10.1631/jzus.B2300738

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利用Bacillus paramycoides细胞内蛋白延伸因子Tu揭示硒纳米颗粒的创新绿色合成机制

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Correspondence | Updated：2024-09-20

利用Bacillus paramycoides细胞内蛋白延伸因子Tu揭示硒纳米颗粒的创新绿色合成机制
Unveiling the innovative green synthesis mechanism of selenium nanoparticles by exploiting intracellular protein elongation factor Tu from Bacillus paramycoides
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2024年25卷第9期页码：789-795
Affiliations：

1.Faculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an 223003, China
2.Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huai’an 223003, China
Funds：

the National Natural Science Foundation of China(32202158);the Natural Science Foundation of Jiangsu Province, China(BK20220703);the Undergraduate Innovation & Entrepreneurship Training Program of Jiangsu Province(202311049168XJ)
DOI：10.1631/jzus.B2300738
中图分类号：
CSTR：
纸质出版日期： 2024-09-15 ，

网络出版日期： 2024-08-19 ，

收稿日期： 2023-10-22 ，

修回日期： 2023-12-26 ，
Accepted：

刘培,龙海钰,贺帅等.利用Bacillus paramycoides细胞内蛋白延伸因子Tu揭示硒纳米颗粒的创新绿色合成机制[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2024,25(09):789-795.

Pei LIU, Haiyu LONG, Shuai HE, et al. Unveiling the innovative green synthesis mechanism of selenium nanoparticles by exploiting intracellular protein elongation factor Tu from Bacillus paramycoides[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2024,25(9):789-795.
刘培,龙海钰,贺帅等.利用Bacillus paramycoides细胞内蛋白延伸因子Tu揭示硒纳米颗粒的创新绿色合成机制[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2024,25(09):789-795. DOI： 10.1631/jzus.B2300738.

Pei LIU, Haiyu LONG, Shuai HE, et al. Unveiling the innovative green synthesis mechanism of selenium nanoparticles by exploiting intracellular protein elongation factor Tu from Bacillus paramycoides[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2024,25(9):789-795. DOI： 10.1631/jzus.B2300738.

摘要

纳米硒（SeNPs）因具有独特特性而备受关注，在各领域均有较好的应用前景。因此，开发高效、环保的SeNPs合成方法具有重要意义。本实验室保存的

Bacillus paramycoides

24522在24 h内可将亚硒酸盐还原为具有高稳定性和高分散性的SeNPs，其平均直径为150 nm（100~200 nm），zeta电位为-29.9 eV。本研究提出了一种绿色新颖的还原机制，即利用

B. paramycoides

24522中细胞质蛋白延伸因子Tu（EF-Tu）还原合成SeNPs，并经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和液相色谱串联质谱（LC-MS/MS）分析，鉴定EF-Tu分子质量约为43 kDa，含395个氨基酸。实时荧光定量聚合酶链反应（qPCR）结果显示EF-Tu的信使RNA（mRNA）表达量增加8.9倍，这进一步证实了EF-Tu参与了亚硒酸盐的还原。透射电镜（TEM）成像结果显示，SeNPs的合成发生在细胞内。综上可知，小SeNPs最初在细胞内作为种子与EF-Tu相互作用，随后经历成熟过程，并最终通过细胞裂解释放到培养基中。这种创新的绿色合成机制为SeNPs的生产提供了宽广的前景，有助于推进环境友好型纳米材料的合成。

Abstract

关键词

纳米硒（SeNPs）副覃状芽孢杆菌（Bacillus paramycoides）延伸因子Tu硒还原绿色合成机制

Keywords

references

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利用Bacillus paramycoides细胞内蛋白延伸因子Tu揭示硒纳米颗粒的创新绿色合成机制

Unveiling the innovative green synthesis mechanism of selenium nanoparticles by exploiting intracellular protein elongation factor Tu from Bacillus paramycoides

DOI：10.1631/jzus.B2300738

摘要

Abstract

关键词

Keywords

references