纤维蛋白通过促进重组酶RAD51在DNA损伤位点的招募参与同源重组修复

慕艳华; 韩金花; 邬明杰; 李宗芳; 杜轲; 魏雅萌; 吴梦婕; 黄俊

doi:10.1631/jzus.B2300518

Your Location：

Home >

Browse articles >

纤维蛋白通过促进重组酶RAD51在DNA损伤位点的招募参与同源重组修复

Scan for full text

文章被引用时，请邮件提醒。

Submit

Correspondence | Updated：2024-01-19

纤维蛋白通过促进重组酶RAD51在DNA损伤位点的招募参与同源重组修复
Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2023年24卷第12期页码：1165-1173
Affiliations：

1.National-Local Joint Engineering Research Center of Biodiagnosis & Biotherapy, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China
2.The MOE Key Laboratory of Biosystems Homeostasis & Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute, Zhejiang University, Hangzhou 310058, China
3.Zhejiang Provincial Key Lab of Geriatrics and Geriatrics Institute of Zhejiang Province, Department of Geriatrics, Zhejiang Hospital, Hangzhou 310030, China
4.Trauma Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
5.The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China
6.Zhejiang Provincial Key Laboratory of Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou 310058, China
7.Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China
Funds：

the National Key Research and Development Program of China(2022YFA1302800;2021YFA1101000);the National Natural Science Foundation of China(31961160725;31730021;31971220;32270769;32170730);the Fok Ying Tung Education Foundation, the Fundamental Research Funds for the Provincial Universities of Zhejiang(2021XZZX039);the Natural Science Foundation of Shaanxi Province(2023-JC-QN-0942);the Research Fund of Xi’an Jiaotong University(YXJLRH2022098);the Research Fund of the Second Affiliated Hospital of Xi’an Jiaotong University (Nos
DOI：10.1631/jzus.B2300518
中图分类号：
CSTR：

慕艳华,韩金花,邬明杰等.纤维蛋白通过促进重组酶RAD51在DNA损伤位点的招募参与同源重组修复[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2023,24(12):1165-1173.

Yanhua MU, Jinhua HAN, Mingjie WU, et al. Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2023,24(12):1165-1173.
慕艳华,韩金花,邬明杰等.纤维蛋白通过促进重组酶RAD51在DNA损伤位点的招募参与同源重组修复[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2023,24(12):1165-1173. DOI： 10.1631/jzus.B2300518.

Yanhua MU, Jinhua HAN, Mingjie WU, et al. Fibrillarin promotes homologous recombination repair by facilitating the recruitment of recombinase RAD51 to DNA damage sites[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2023,24(12):1165-1173. DOI： 10.1631/jzus.B2300518.

摘要

同源重组（HR）是一种高度精确的DNA双链断裂（DSB）损伤修复方式。HR修复的关键步骤是重组酶RAD51通过包裹单链DNA形成核酸蛋白纤维丝，进行同源模板搜索，并进行DNA链入侵反应，从而启动DNA修复合成。本研究发现，纤维蛋白（FBL）是重要的HR调节因子。一旦发生DNA损伤，FBL就被招募到DSB位点，并直接与RAD51互作。细胞缺失FBL会导致RAD51在DNA损伤位点募集减少，HR修复效率降低。此外，细胞缺失FBL会导致染色体畸变增加，促使细胞对DNA损伤药物敏感。本研究提出了FBL介导的RAD51在DNA损伤位点的招募的新机制，并强调了FBL在癌症治疗中的潜在意义。

Abstract

关键词

基因组不稳定性DNA双链断裂同源重组RAD51纤维蛋白

Keywords

references

Blunt T, Finnie NJ, Taccioli GE, et al., 1995. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell, 80(5):813-823. https://doi.org/10.1016/0092-8674(95)90360-7https://doi.org/10.1016/0092-8674(95)90360-7

Canela A, Maman Y, Jung S, et al., 2017. Genome organization drives chromosome fragility. Cell, 170(3):507-521.e18. https://doi.org/10.1016/j.cell.2017.06.034https://doi.org/10.1016/j.cell.2017.06.034

Hanahan D, 2022. Hallmarks of cancer: new dimensions. Cancer Discov, 12(1):31-46. https://doi.org/10.1158/2159-8290.CD-21-1059https://doi.org/10.1158/2159-8290.CD-21-1059

Hartley KO, Gell D, Smith GCM, et al., 1995. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell, 82(5):849-856. https://doi.org/10.1016/0092-8674(95)90482-4https://doi.org/10.1016/0092-8674(95)90482-4

Hilario J, Amitani I, Baskin RJ, et al., 2009. Direct imaging of human Rad51 nucleoprotein dynamics on individual DNA molecules. Proc Natl Acad Sci USA, 106(2):361-368. https://doi.org/10.1073/pnas.0811965106https://doi.org/10.1073/pnas.0811965106

Katsuki Y, Jeggo PA, Uchihara Y, et al., 2020. DNA double-strand break end resection: a critical relay point for determining the pathway of repair and signaling. Genome Instab Dis, 1(4):155-171. https://doi.org/10.1007/s42764-020-00017-8https://doi.org/10.1007/s42764-020-00017-8

Li YH, Yuan J, 2021. Role of deubiquitinating enzymes in DNA double-strand break repair. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 22(1):63-72. https://doi.org/10.1631/jzus.B2000309https://doi.org/10.1631/jzus.B2000309

Ma CJ, Gibb B, Kwon Y, et al., 2017. Protein dynamics of human RPA and RAD51 on ssDNA during assembly and disassembly of the RAD51 filament. Nucleic Acids Res, 45(2):749-761. https://doi.org/10.1093/nar/gkw1125https://doi.org/10.1093/nar/gkw1125

Marcel V, Ghayad SE, Belin S, et al., 2013. p53 acts as a safeguard of translational control by regulating fibrillarin and rRNA methylation in cancer. Cancer Cell, 24(3):318-330. https://doi.org/10.1016/j.ccr.2013.08.013https://doi.org/10.1016/j.ccr.2013.08.013

Miné J, Disseau L, Takahashi M, et al., 2007. Real-time measurements of the nucleation, growth and dissociation of single Rad51-DNA nucleoprotein filaments. Nucleic Acids Res, 35(21):7171-7187. https://doi.org/10.1093/nar/gkm752https://doi.org/10.1093/nar/gkm752

Newton K, Petfalski E, Tollervey D, et al., 2003. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol, 23(23):8519-8527. https://doi.org/10.1128/MCB.23.23.8519-8527.2003https://doi.org/10.1128/MCB.23.23.8519-8527.2003

Omer AD, Ziesche S, Ebhardt H, et al., 2002. In vitro reconstitution and activity of a C/D box methylation guide ribonucleoprotein complex. Proc Natl Acad Sci USA, 99(8):5289-5294. https://doi.org/10.1073/pnas.082101999https://doi.org/10.1073/pnas.082101999

Schimmang T, Tollervey D, Kern H, et al., 1989. A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability. EMBO J, 8(13):4015-4024. https://doi.org/10.1002/j.1460-2075.1989.tb08584.xhttps://doi.org/10.1002/j.1460-2075.1989.tb08584.x

Shinohara A, Ogawa H, Ogawa T, 1992. Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell, 69(3):457-470. https://doi.org/10.1016/0092-8674(92)90447-Khttps://doi.org/10.1016/0092-8674(92)90447-K

Snaar S, Wiesmeijer K, Jochemsen AG, et al., 2000. Mutational analysis of fibrillarin and its mobility in living human cells. J Cell Biol, 151(3):653-662. https://doi.org/10.1083/jcb.151.3.653https://doi.org/10.1083/jcb.151.3.653

Tessarz P, Santos-Rosa H, Robson SC, et al., 2014. Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification. Nature, 505(7484):564-568. https://doi.org/10.1038/nature12819https://doi.org/10.1038/nature12819

Tiku V, Jain C, Raz Y, et al., 2017. Small nucleoli are a cellular hallmark of longevity. Nat Commun, 8:16083. https://doi.org/10.1038/ncomms16083https://doi.org/10.1038/ncomms16083

Tollervey D, Lehtonen H, Jansen R, et al., 1993. Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly. Cell, 72(3):443-457. https://doi.org/10.1016/0092-8674(93)90120-Fhttps://doi.org/10.1016/0092-8674(93)90120-F

Xiang ZY, Liu H, Hu Y, 2023. DNA damage repair and cancer immunotherapy. Genome Instab Dis, 4(4):210-226. https://doi.org/10.1007/s42764-023-00098-1https://doi.org/10.1007/s42764-023-00098-1

Yi YH, Ma TH, Lee LW, et al., 2015. A genetic cascade of let-7-ncl-1-fib-1 modulates nucleolar size and rRNA pool in Caenorhabditis elegans. PLoS Genet, 11(10):e1005580. https://doi.org/10.1371/journal.pgen.1005580https://doi.org/10.1371/journal.pgen.1005580

Yoshikawa H, Komatsu W, Hayano T, et al., 2011. Splicing factor 2-associated protein p32 participates in ribosome biogenesis by regulating the binding of Nop52 and fibrillarin to preribosome particles. Mol Cell Proteomics, 10(8):M110.006148. https://doi.org/10.1074/mcp.M110.006148https://doi.org/10.1074/mcp.M110.006148

Zhang F, Gong ZH, 2021. Regulation of DNA double-strand break repair pathway choice: a new focus on 53BP1. J Zhejiang Univ-Sci B (Biomed & Biotechnol), 22(1):38-46. https://doi.org/10.1631/jzus.B2000306https://doi.org/10.1631/jzus.B2000306

浏览量

Downloads

CSCD

工具集

关联资源

Construction of recombinant industrial Saccharomyces cerevisiae strain with bglS gene insertion into PEP4 locus by homologous recombination