光遗传学在口腔和颌面部研究中的应用

张芩梦; 宋璐瑶; 傅梦蝶; 贺瑾; 杨国利; 姜治伟

doi:10.1631/jzus.B2300322

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Review | Updated：2024-08-19

光遗传学在口腔和颌面部研究中的应用
Optogenetics in oral and craniofacial research
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2024年25卷第8期页码：656-671
Affiliations：

1.Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310000, China
2.Zhejiang University School of Medicine, Hangzhou 310058, China
Funds：

the National Natural Science Foundation of China(82370991);the Health Department of Zhejiang Province(2021KY194;2022KY872);ChinaAcknowledgement:
DOI：10.1631/jzus.B2300322
中图分类号：
CSTR：
纸质出版日期： 2024-08-15 ，

收稿日期： 2023-05-10 ，

修回日期： 2023-10-17 ，
Accepted：

张芩梦,宋璐瑶,傅梦蝶等.光遗传学在口腔和颌面部研究中的应用[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2024,25(08):656-671.

Qinmeng ZHANG, Luyao SONG, Mengdie FU, et al. Optogenetics in oral and craniofacial research[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2024,25(8):656-671.
张芩梦,宋璐瑶,傅梦蝶等.光遗传学在口腔和颌面部研究中的应用[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2024,25(08):656-671. DOI： 10.1631/jzus.B2300322.

Qinmeng ZHANG, Luyao SONG, Mengdie FU, et al. Optogenetics in oral and craniofacial research[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2024,25(8):656-671. DOI： 10.1631/jzus.B2300322.

摘要

光遗传学通过光学和基因工程相结合对特定的基因表达和生物功能进行控制，具有精确的时空控制、无创和高效等优点。蛋白质被植入经基因改造的光感传感器后，在光刺激下调节构象的变化。因此，光遗传学技术可在从亚细胞和细胞水平到神经回路和行为模型等不同层面上为口腔生物学研究提供新的见解。本综述介绍了光遗传学的起源和光遗传学方法在口腔颌面研究中的最新进展，着重描述了通道视紫红质（ChR）、古紫质（Arch）和氯视紫红质（NpHR）等光遗传学工具在神经科学基础机制研究中的应用，以及在体内建立不同的口腔行为测试模型（口面部运动、舔舐、进食和饮水），同时回顾了光遗传学在三叉神经痛和颌面蜂窝织炎的临床前研究中的协同和拮抗作用。此外，在转化研究中，光遗传学工具被用于控制牙髓干细胞的神经源性分化。虽然光遗传学工具的应用范围在不断扩大，但其在口腔研究领域的大型动物实验和临床研究中的应用还很有限。光遗传学潜在的应用方向包括探索2019冠状病毒病（COVID-19）患者味觉丧失的治疗策略、研究口腔细菌生物膜、增强颅颌面和牙周组织再生，以及阐明干槽症、口干症和灼口综合征的可能发病机制。

Abstract

Optogenetics combines optics and genetic engineering to control specific gene expression and biological functions and has the advantages of precise spatiotemporal control

noninvasiveness

and high efficiency. Genetically modified photosensory sensors are engineered into proteins to modulate conformational changes with light stimulation. Therefore

optogenetic techniques can provide new insights into oral biological processes at different levels

ranging from the subcellular and cellular levels to neural circuits and behavioral models. Here

we introduce the origins of optogenetics and highlight the recent progress of optogenetic approaches in oral and craniofacial research

focusing

on the ability to apply optogenetics to the study of basic scientific neural mechanisms and to establish different oral behavioral test models in vivo (orofacial movement

licking

eating

and drinking)

such as channelrhodopsin (ChR)

archaerhodopsin (Arch)

and halorhodopsin from

Natronomonas pharaonis

(NpHR). We also review the synergic and antagonistic effects of optogenetics in preclinical studies of trigeminal neuralgia and maxillofacial cellulitis. In addition

optogenetic tools have been used to control the neurogenic differentiation of dental pulp stem cells in translational studies. Although the scope of optogenetic tools is increasing

there are limited large animal experiments and clinical studies in dental research. Potential future directions include exploring therapeutic strategies for addressing loss of taste in patients with coronavirus disease 2019 (COVID-19)

studying oral bacterial biofilms

enhancing craniomaxillofacial and periodontal tissue regeneration

and elucidating the possible pathogenesis of dry sockets

xerostomia

and burning mouth syndrome.

关键词

激光细胞分化细菌毒力神经系统神经生理学行为科学

Keywords

LasersCell differentiationBacterial virulenceNervous systemNeurophysiologyBehavioral science

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