神经肽受体Y8b（<italic style="font-style: italic">npy8br</italic>）调节日本青鳉（<italic style="font-style: italic">Oryzias latipes</italic>）仔鱼的摄食和消化： 来自基因敲除的证据

贾晓丹; 陆可; 梁旭方

doi:10.1631/jzus.B2300312

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神经肽受体Y8b（npy8br）调节日本青鳉（Oryzias latipes）仔鱼的摄食和消化：来自基因敲除的证据

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Research Article | Updated：2024-07-17

神经肽受体Y8b（npy8br）调节日本青鳉（Oryzias latipes）仔鱼的摄食和消化：来自基因敲除的证据
Neuropeptide Y receptor Y8b (npy8br) regulates feeding and digestion in Japanese medaka (Oryzias latipes) larvae: evidence from gene knockout
浙江大学学报（英文版）（B辑：生物医学和生物技术） 2024年25卷第7期页码：605-616
Affiliations：

1.College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China
2.Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, China
Funds：

the National Natural Science Foundation of China(31972809);the Key Research & Development Program of Hubei Province(2022BBA0051)
DOI：10.1631/jzus.B2300312
中图分类号：
CSTR：
纸质出版日期： 2024-07-15 ，

网络出版日期： 2024-06-05 ，

收稿日期： 2023-05-09 ，

修回日期： 2023-09-05 ，
Accepted：

贾晓丹,陆可,梁旭方.神经肽受体Y8b（npy8br）调节日本青鳉（Oryzias latipes）仔鱼的摄食和消化：来自基因敲除的证据[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2024,25(07):605-616.

Xiaodan JIA, Ke LU, Xufang LIANG. Neuropeptide Y receptor Y8b (npy8br) regulates feeding and digestion in Japanese medaka (Oryzias latipes) larvae: evidence from gene knockout[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2024,25(7):605-616.
贾晓丹,陆可,梁旭方.神经肽受体Y8b（npy8br）调节日本青鳉（Oryzias latipes）仔鱼的摄食和消化：来自基因敲除的证据[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2024,25(07):605-616. DOI： 10.1631/jzus.B2300312.

Xiaodan JIA, Ke LU, Xufang LIANG. Neuropeptide Y receptor Y8b (npy8br) regulates feeding and digestion in Japanese medaka (Oryzias latipes) larvae: evidence from gene knockout[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2024,25(7):605-616. DOI： 10.1631/jzus.B2300312.

摘要

神经肽Y受体Y8（NPY8R）是一种鱼类特异性受体，具有NPY8AR和NPY8BR两种亚型。生理过程中表达水平的变化以及心室注射后体内调节过程表明，NPY8BR在摄食调节中发挥着重要作用；目前只在少数鱼类中发现有这种作用。为了更好地了解NPY8BR的生理功能，特别是消化方面，我们利用CRISPR/Cas9技术构建了

npy8br

-/-

日本青鳉（

Oryzias latipes

）。实验结果表明，

npy8br

基因缺失会影响青鳉仔鱼的摄食和消化能力，并最终影响其生长。具体来说，缺失

npy8br

会导致鳉鱼仔鱼摄食量减少和食欲相关基因（

npy

、

agrp

）表达水平降低。饲喂10 d（饲喂第10天）的

npy8br

-/-

青鳉仔鱼在摄食8 h后消化道内仍有未完全消化的卤虫（

Artemia nauplii

），消化相关基因（

amy

、

lpl

、

ctra

和

ctrb

）mRNA表达量显著降低，且淀粉酶、胰蛋白酶和脂肪酶活性也显著降低。

npy8br

的缺失抑制了青鳉仔鱼的生长，显著降低了生长相关基因（

和

igf1

）的表达。肠道组织切片显示，

npy8br

-/-

青鳉仔鱼肠道受损，肠壁变薄，肠绒毛变短。本研究首次成功构建了鱼类的

npy8br

基因敲除模型，并证明

npy8br

在消化过程发挥着重要作用。

Abstract

Neuropeptide Y receptor Y8 (NPY8R) is a fish-specific receptor with two subtypes

NPY8AR and NPY8BR. Changes in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation; this has been found in only a few fish

at present. In order to better understand the physiological function of

npy8br

especially in digestion

we used clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology to generate

npy8br

-/-

Japanese medaka (

Oryzias latipes

). We found that the deletion of

npy8br

in medaka larvae affected their feeding and digestion ability

ultimately affecting their growth. Specifically

npy8br

deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes (

npy

and

agrp

npy8br

-/-

medaka larvae fed for 10 d (10th day of feeding) still had incompletely digested brine shrimp (

Artemia nauplii

) in the digestive tract 8 h after feeding

the messenger RNA (mRNA) expression levels of digestion-related genes (

amy

lpl

ctra

and

ctrb

) were significantly decreased

and the activity of amylase

trypsin

and lipase also significantly decreased. The deletion of

npy8br

in medaka larvae inhibited the growth and significantly decrease

d the expression of growth-related genes (

and

igf1

). Hematoxylin and eosin (H

E) sections of intestinal tissue showed that

npy8br

-/-

medaka larvae had damaged intestine

thinned intestinal wall

and shortened intestinal villi. So far

this is the first

npy8br

gene knockout model established in fish and the first demonstration that

npy8br

plays an important role in digestion.

关键词

神经肽Y受体Y8（npy8br）日本青鳉(Oryzias latipes)敲除摄食消化

Keywords

Neuropeptide Y receptor Y8b (npy8br)Japanese medaka (Oryzias latipes)KnockoutFeedingDigestion

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神经肽受体Y8b（npy8br）调节日本青鳉（Oryzias latipes）仔鱼的摄食和消化： 来自基因敲除的证据

Neuropeptide Y receptor Y8b (npy8br) regulates feeding and digestion in Japanese medaka (Oryzias latipes) larvae: evidence from gene knockout

DOI：10.1631/jzus.B2300312

摘要

Abstract

关键词

Keywords

references

神经肽受体Y8b（npy8br）调节日本青鳉（Oryzias latipes）仔鱼的摄食和消化：来自基因敲除的证据