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Effects of
Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickensEffects of
Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickensAeriscardovia aeriphila 对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响- Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) Vol. 24, Issue 11, Pages: 1014-1026(2023)
- Affiliations：
1.National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Frontiers Science Center for Animal Breeding and Sustainable Production, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China
2.Department of Animal Sciences, University of Veterinary and Animal Sciences (Jhang Campus), Lahore 54000, Pakistan
- Funds：
DOI：10.1631/jzus.B2200621
CLC：- CSTR：
Muhammad Zahid FAROOQ,王新锴,晏向华.,Aeriscardovia aeriphila对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2023,24(11):1014-1026.
Muhammad Zahid FAROOQ, Xinkai WANG, Xianghua YAN. Effects of
Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens. [J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) 24(11):1014-1026(2023)Muhammad Zahid FAROOQ,王新锴,晏向华.,Aeriscardovia aeriphila对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2023,24(11):1014-1026. DOI： 10.1631/jzus.B2200621.
Muhammad Zahid FAROOQ, Xinkai WANG, Xianghua YAN. Effects of
Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens. [J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) 24(11):1014-1026(2023) DOI： 10.1631/jzus.B2200621.
摘要
Aeriscardovia aeriphila,，又名,Bifidobacterium aerophilum,，是从猪盲肠内容物中分离出的一种双歧杆菌。,A. aeriphila,在预防肠道感染、降低胆固醇水平和刺激免疫系统方面具有重要作用。本研究首先从肉鸡十二指肠肠道内容物中分离出一株菌， 经16S 测序鉴定为 ,A. aeriphila,，然后我们评估了,A. aeriphila,对商用肉鸡生长性能、抗氧化功能、免疫功能和肠道菌群的影响。实验对21日龄的实验鸡进行了为期3周的灌服,A. aeriphila,（1×10,9, CFU/mL）处理。研究结果显示：,A. aeriphila,处理显著增加了鸡的平均日增重，降低了饲料转化率（,P,<,0.001）。,A. aeriphila,处理后，血清生长激素（GH）和胰岛素样生长因子1（IGF-1）水平显著升高（,P,<,0.05）。,A. aeriphila,处理降低了血尿素氮和天门冬氨酸氨基转移酶水平，增加了血糖和肌酐水平。同时，,A. aeriphila,处理显著提高了血清中抗氧化酶水平（包括过氧化氢酶（,P,<,0.01）、超氧化物歧化酶（,P,<,0.001）和谷胱甘肽过氧化物酶（,P,<,0.05））及总抗氧化能力（,P,<,0.05）。,A. aeriphila,处理显著增加了血清免疫球蛋白A（IgA）（,P,<,0.05）、IgG（,P,<,0.01）、IgM（,P,<,0.05）、白细胞介素-1（IL-1）（,P,<,0.05）、IL-4（,P,<,0.05）和IL-10（,P,<,0.05）的水平。与对照组相比，,A. aeriphila,组肉鸡在十二指肠（,P,<,0.01）、空肠（,P,<,0.001）和盲肠（,P,<,0.001）中的分泌型免疫球蛋白A（SIgA）水平显著提高。在肠道黏膜（包括十二指肠、空肠和盲肠）中，,A. aeriphila,组鸡的,IL-10,（,P,<,0.05）、,IL-4,（,P,<,0.001）和,NF-κB,（,P,<,0.001）信使RNA（mRNA）相对表达水平高于对照组。肠道微生物门水平分析显示，两组的差异菌群主要是厚壁菌门，其次是拟杆菌门。此外，,A. aeriphila,可提高肠道中,Phascolarctobacterium,和,Barnesiella,的丰度。综上所述，灌服,A. aeriphila,可改善肉鸡的生长性能、血清抗氧化能力、免疫调节和肠道健康。因此，本研究结果可以为,A. aeriphila,在家禽生产中的应用提供理论依据。
Abstract
Aeriscardovia aeriphila, also known as ,Bifidobacterium aerophilum, was first isolated from the caecal contents of pigs and the faeces of cotton-top tamarin. ,Bifidobacterium, species play important roles in preventing intestinal infections, decreasing cholesterol levels, and stimulating the immune system. In this study, we isolated a strain of bacteria from the duodenal contents of broiler chickens, which was identified as ,A. aeriphila, and then evaluated the effects of ,A. aeriphila, on growth performance, antioxidant functions, immune functions, and gut microbiota in commercial broiler chickens. Chickens were orally gavaged with ,A. aeriphila, (1×10,9, CFU/mL) for 21 d. The results showed that ,A. aeriphila, treatment significantly increased the average daily gain and reduced the feed conversion ratio (,P,<,0.001). The levels of serum growth hormone (GH) and insulin-like growth factor 1 (IGF-1) were significantly increased following ,A. aeriphila, treatment (,P,<,0.05). Blood urea nitrogen and aspartate aminotransferase levels were decreased, whereas glucose and creatinine levels increased as a result of ,A. aeriphila, treatment. Furthermore, the levels of serum antioxidant enzymes, including catalase (,P,<,0.01), superoxide dismutase (,P,<,0.001), and glutathione peroxidase (,P,<,0.05), and total antioxidant capacity (,P,<,0.05) were enhanced following ,A. aeriphila, treatment. ,A. aeriphila, treatment significantly increased the levels of serum immunoglobulin A (IgA) (,P,<,0.05), IgG (,P,<,0.01), IgM (,P,<,0.05), interleukin-1 (IL-1) (,P,<,0.05), IL-4 (,P,<,0.05), and IL-10 (,P,<,0.05). The broiler chickens in the ,A. aeriphila, group had higher secretory IgA (SIgA) levels in the duodenum (,P,<,0.01), jejunum (,P,<,0.001), and cecum (,P,<,0.001) than those in the control group. The messenger RNA (mRNA) relative expression levels of ,IL-10 ,(,P,<,0.05) and ,IL-4, (,P,<,0.001) in the intestinal mucosa of chickens were increased, while nuclear factor-κB (,NF,,-,,κB,) (,P,<,0.001) expression was decreased in the ,A. aeriphila, group compared to the control group. Phylum-level analysis revealed Firmicutes as the main phylum, followed by Bacteroidetes, in both groups. The data also found that ,Phascolarctobacterium ,and ,Barnesiella, were increased in ,A. aeriphila,-treated group. In conclusion, oral administration of ,A. aeriphila, could improve the growth performance, serum antioxidant capacity, immune modulation, and gut health of broilers. Our findings may provide important information for the application of ,A. aeriphila ,in poultry production.
关键词
鸡Aeriscardovia aeriphila肠道健康菌群免疫功能
Keywords
ChickenAeriscardovia aeriphilaGut healthMicrobiomeImmune function
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