Effects of <italic style="font-style: italic">Aeriscardovia aeriphila</italic> on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens

Muhammad Zahid FAROOQ; Xinkai WANG; Xianghua YAN

doi:10.1631/jzus.B2200621

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Research Article | Updated：2024-12-02

Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens
Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens
Aeriscardovia aeriphila对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) Vol. 24, Issue 11, Pages: 1014-1026(2023)
Affiliations：

1.National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Frontiers Science Center for Animal Breeding and Sustainable Production, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China
2.Department of Animal Sciences, University of Veterinary and Animal Sciences (Jhang Campus), Lahore 54000, Pakistan
Funds：
DOI：10.1631/jzus.B2200621
CLC：
CSTR：
Published： 15 November 2023 ，

Published Online： 22 September 2023 ，

Received： 14 December 2022 ，

Revised： 16 April 2023 ，
Accepted：

Muhammad Zahid FAROOQ,王新锴,晏向华.,Aeriscardovia aeriphila对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2023,24(11):1014-1026.

MUHAMMAD ZAHID FAROOQ, XINKAI WANG, XIANGHUA YAN. Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens. [J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2023, 24(11): 1014-1026.
Muhammad Zahid FAROOQ,王新锴,晏向华.,Aeriscardovia aeriphila对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,2023,24(11):1014-1026. DOI： 10.1631/jzus.B2200621.

MUHAMMAD ZAHID FAROOQ, XINKAI WANG, XIANGHUA YAN. Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens. [J]. Journal of zhejiang university-science b (biomedicine & biotechnology), 2023, 24(11): 1014-1026. DOI： 10.1631/jzus.B2200621.

摘要

Aeriscardovia aeriphila

，又名

Bifidobacterium aerophilum

，是从猪盲肠内容物中分离出的一种双歧杆菌。

A. aeriphila

在预防肠道感染、降低胆固醇水平和刺激免疫系统方面具有重要作用。本研究首先从肉鸡十二指肠肠道内容物中分离出一株菌，经16S 测序鉴定为

A. aeriphila

，然后我们评估了

A. aeriphila

对商用肉鸡生长性能、抗氧化功能、免疫功能和肠道菌群的影响。实验对21日龄的实验鸡进行了为期3周的灌服

A. aeriphila

（1×10

CFU/mL）处理。研究结果显示：

A. aeriphila

处理显著增加了鸡的平均日增重，降低了饲料转化率（

0.001）。

A. aeriphila

处理后，血清生长激素（GH）和胰岛素样生长因子1（IGF-1）水平显著升高（

0.05）。

A. aeriphila

处理降低了血尿素氮和天门冬氨酸氨基转移酶水平，增加了血糖和肌酐水平。同时，

A. aeriphila

处理显著提高了血清中抗氧化酶水平（包括过氧化氢酶（

0.01）、超氧化物歧化酶（

0.001）和谷胱甘肽过氧化物酶（

0.05））及总抗氧化能力（

0.05）。

A. aeriphila

处理显著增加了血清免疫球蛋白A（IgA）（

0.05）、IgG（

0.01）、IgM（

0.05）、白细胞介素-1（IL-1）（

0.05）、IL-4（

0.05）和IL-10（

0.05）的水平。与对照组相比，

A. aeriphila

组肉鸡在十二指肠（

0.01）、空肠（

0.001）和盲肠（

0.001）中的分泌型免疫球蛋白A（SIgA）水平显著提高。在肠道黏膜（包括十二指肠、空肠和盲肠）中，

A. aeriphila

组鸡的

IL-10

（

0.05）、

IL-4

（

0.001）和

NF-κB

（

0.001）信使RNA（mRNA）相对表达水平高于对照组。肠道微生物门水平分析显示，两组的差异菌群主要是厚壁菌门，其次是拟杆菌门。此外，

A. aeriphila

可提高肠道中

Phascolarctobacterium

和

Barnesiella

的丰度。综上所述，灌服

A. aeriphila

可改善肉鸡的生长性能、血清抗氧化能力、免疫调节和肠道健康。因此，本研究结果可以为

A. aeriphila

在家禽生产中的应用提供理论依据。

Abstract

Aeriscardovia aeriphila

also known as

Bifidobacterium aerophilum

was first isolated from the caecal contents of pigs and the faeces of cotton-top tamarin.

Bifidobacterium

species play important roles in preventing intestinal infections

decreasing cholesterol levels

and stimulating the immune system. In this study

we isolated a strain of bacteria from the duodenal contents of broiler chickens

which was identified as

A. aeriphila

and then evaluated the effects of

A. aeriphila

on growth performance

antioxidant functions

immune functions

and gut microbiota in commercial broiler chickens. Chickens were orally gavaged with

A. aeriphila

(1×10

CFU/mL) for 21 d. The results showed that

A. aeriphila

treatment significantly increased the average daily gain and reduced the feed conversion ratio (

0.001). The levels of serum growth hormone (GH) and insulin-like growth factor 1 (IGF-1) were significantly increased following

A. aeriphila

treatment (

0.05). Blood urea nitrogen and aspartate aminotransferase levels were decreased

whereas glucose and creatinine levels increased as a result of

A. aeriphila

treatment. Furthermore

the levels of serum antioxidant enzymes

including catalase (

0.01)

superoxide dismutase (

0.001)

and glutathione peroxidase (

0.05)

and total antioxidant capacity (

0.05) were enhanced following

A. aeriphila

treatment.

A. aeriphila

treatment significantly increased the levels of serum

immunoglobulin A (IgA) (

0.05)

IgG (

0.01)

IgM (

0.05)

interleukin-1 (IL-1) (

0.05)

IL-4 (

0.05)

and IL-10 (

0.05). The broiler chickens in the

A. aeriphila

group had higher secretory IgA (SIgA) levels in the duodenum (

0.01)

jejunum (

0.001)

and cecum (

0.001) than those in the control group. The messenger RNA (mRNA) relative expression levels of

IL-10

(

0.05) and

IL-4

(

0.001) in the intestinal mucosa of chickens were increased

while nuclear factor-‍κB (

‍

κB

) (

0.001) expression was decreased in the

A. aeriphila

group compared to the control group. Phylum-level analysis revealed Firmicutes as the main phylum

followed by Bacteroidetes

in both groups. The data also found that

Phascolarctobacterium

and

Barnesiella

were increased in

A. aeriphila

-treated group. In conclusion

oral administration of

A. aeriphila

could improve the growth performance

serum antioxidant capacity

immune modulation

and gut health of broilers. Our findings may provide important information for the application of

A. aeriphila

in poultry production.

关键词

鸡Aeriscardovia aeriphila肠道健康菌群免疫功能

Keywords

ChickenAeriscardovia aeriphilaGut healthMicrobiomeImmune function

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⁰

Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens

Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens

DOI：10.1631/jzus.B2200621

摘要

Abstract

关键词

Keywords

references