Antioxidant and antidiabetic properties of tartary buckwheat rice flavonoids after in vitro digestion

Antioxidant and antidiabetic properties of tartary buckwheat rice flavonoids after in vitro digestion 体外模拟消化对黑苦荞米黄酮抗氧化及降血糖活性的影响 1 first-author 3 1 1 3 3 3 1 corresp ‡Corresponding Authors ‡Corresponding Authors †E-mail:zjuchenwei@zju.edu.cnadesun2006@zju.edu.cn †E-mail:zjuchenwei@zju.edu.cnadesun2006@zju.edu.cn zjuchenwei@zju.edu.cn 2 corresp ‡Corresponding Authors ‡Corresponding Authors †E-mail:zjuchenwei@zju.edu.cnadesun2006@zju.edu.cn †E-mail:zjuchenwei@zju.edu.cnadesun2006@zju.edu.cn zjuchenwei@zju.edu.cn Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China Laboratory of Fruit Quality Biology, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, the State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, China Laboratory of Fruit Quality Biology, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, the State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, China Nutrition and Health Research Institute, COFCO Ltd., Beijing 102209, China Nutrition and Health Research Institute, COFCO Ltd., Beijing 102209, China Oxidative stress and diabetes have a tendency to alter protein, lipid, and DNA moieties. One of the strategic methods used to reduce diabetes-associated oxidative stress is to inhibit the carbohydrate-digesting enzymes, thereby decreasing gastrointestinal glucose production. Plant-derived natural antioxidant molecules are considered a therapeutic tool in the treatment of oxidative stress and diabetes. The objective of this study was to identify tartary buckwheat rice flavonoids and evaluate the effect of in vitro digestion on their antioxidant and antidiabetic properties. High performance liquid chromatography (HPLC) analysis indicated the presence of rutin as a major component and quercitrin as a minor component of both digested and non-digested flavonoids. Both extracts showed a significant antioxidant capacity, but digested flavonoids showed reduced activity compared to non-digested. There were some decreases of the antioxidant activities (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazy (DPPH) radical, and ferric reducing antioxidant power (FRAP)) of digested tartary buckwheat rice flavonoids compared with non-digested. Flavonoids from both groups significantly inhibited reactive oxygen species (ROS) production and α-glucosidase activity. Both digested and non-digested flavonoids markedly increased glucose consumption and glycogen content in HepG2 cells. Tartary buckwheat rice flavonoids showed appreciable antioxidant and antidiabetic properties, even after digestion. Tartary buckwheat rice appears to be a promising functional food with potent antioxidant and antidiabetic properties. 目的 2 研究黑苦荞米黄酮模拟消化前后活性成分含量变化，以及模拟消化对其抗氧化及降血糖活性的影响。 创新点 2 采用体外模拟消化的方法，更加真实地反应消化对黑苦荞米黄酮含量及活性的影响，同时采用体外抗氧化方法、α-葡萄糖苷酶实验和HepG2细胞模型，评价其抗氧化和降血糖活性。 方法 2 本文采用体外模拟消化的方法，对黑苦荞米黄酮进行模拟消化，使用高效液相色谱（HPLC）检测其消化前后黄酮物质含量变化情况（表1和图1）；通过ABTS、DPPH和FRAP等实验研究模拟消化对其抗氧化活性的影响（表2和图2），进一步检测其消化前后对细胞活性氧（ROS）产生的抑制能力（图3）；利用α-葡萄糖苷酶活性抑制实验（图4）以及细胞葡萄糖消耗和糖原含量实验（图5），评价黑苦荞米黄酮模拟消化前后的降血糖活性。 结论 2 HPLC结果表明，黑苦荞米黄酮中的主要活性物质为芦丁和槲皮苷，经模拟消化后，其活性物质含量没有显著变化。体外抗氧化实验ABTS、DPPH和FRAP结果均表明，黑苦荞米黄酮具有抗氧化活性，体外模拟消化后，其抗氧化活性有所降低；细胞ROS结果表明，黑苦荞米黄酮模拟消化前后均具有较好的ROS抑制活性。进一步研究表明，黑苦荞米黄酮模拟消化前后对α-葡萄糖苷酶具有较好的抑制能力，同时可以促进细胞对葡萄糖的消耗以及细胞糖原的生成，具有较好的降血糖活性。 Tartary buckwheat rice Flavonoids In vitro digestion Antioxidant activity Antidiabetic activity 黑苦荞米 黄酮 体外模拟消化 抗氧化 降血糖 Introduction 1 Oxidative stress instigated by reactive oxygen species (ROS) leads to mutations and DNA damage (Chen et al., 2015 2015 ), which further leads to the development of chronic disorders and age-related diseases (Khansari et al., 2009 2009 ; Klaunig et al., 2010 2010 ; Chen et al., 2012b 2012b ; Rahman et al., 2012 2012 ). Oxidative stress-induced cellular dysfunction occurs when endogenous antioxidant defence mechanisms and DNA repair systems are overwhelmed. Hence, oxidative stress plays a critical role in the manifestation of micro- and macro-complications of diabetes by modifying protein, lipid, and DNA moieties (Giacco and Brownlee, 2010 2010 ; Su et al., 2016 2016 ). Diabetes is characterised by an increase in blood glucose (Gowd and Nandini, 2015 2015 ) and is associated with multiple etiologies including interruptions to carbohydrate, fat, and protein metabolism (Liu et al., 2013 2013 ). Diabetes can be managed by reducing oxidative stress. Antioxidants are known to reduce the complications of diabetes induced by oxidative stress by delaying glucose absorption. This is achieved by inhibiting carbohydrate hydrolyzing enzymes such as α-amylase and α-glucosidase (Yu et al., 2012 2012 ; Alakolanga et al., 2015 2015 ). α-Amylase, a primary enzyme mostly found in the saliva, is capable of decomposing starch into absorbable molecules (Kim et al., 2014 2014 ). α-Glucosidase is located in the mucosal brush border and helps the digestion of oligosaccharides to monosaccharides (Hlila et al., 2015 2015 ). There is increasing interest in finding enzyme inhibitors which delay carbohydrate breakdown and eliminate the postprandial peak in blood glucose (Kwon et al., 2007 2007 ). Studies have shown that most natural antioxidants can enhance the action of defence systems against oxidative stress and the management of postprandial hyperglycaemia by inhibiting primary carbohydrate hydrolyzing enzymes such as α-amylase and α-glucosidase (Hashim et al., 2013 2013 ). Due to the presence of a wide range of bioactive compounds with potent antioxidant (Chen et al., 2011 2011 ; 2013a 2013a ) and antidiabetic properties (Khansari et al., 2009 2009 ; Hashim et al., 2013 2013 ), plant products are of great interest. Tartary buckwheat (TBW), Fagopyrum tataricum , a member of the Polygonaceae, is found all over the world, especially in China and Russia (Li and Zhang, 2001 2001 ). Globally, it is used as a human dietary supplement in a form of bran, groats, and flour. TBW is also used as a substitute for rice in some parts of the world and especially in China. Extensive research has reported the potential health benefits of buckwheat, especially its use in Chinese traditional medicine against various cancers (Zhou et al., 2012 2012 ). Consumption of buckwheat and buckwheat-enriched diets is associated with a myriad of biological activities, such as antidiabetic (Lee et al., 2007 2007 ; Hosaka et al., 2011 2011 ), anti-hypercholesterolemic (Choi et al., 2007 2007 ), anti-oxidative, anti-hypertensive (Merendino et al., 2014 2014 ), anti-cancer, and anti-inflammatory properties (Lee et al., 2013 2013 ; Zhu, 2016 2016 ). Researchers have speculated that these properties could be linked to the potential antioxidant activity of compounds in such diets. Oxidative stress is notorious for its decisive role in the development of various kinds of metabolic disorders and other degenerative diseases. The antioxidant capacity of buckwheat has been widely studied (Kreft, 2016 2016 ). Flavonoids in buckwheat have been shown to reduce high glucose-induced oxidative stress. Supplementation of hepatic cells with TBW flavonoids provided protection against high glucose-induced oxidative stress and insulin resistance via MAPK signalling (Hu et al., 2016 2016 ). In another study, supplementation with buckwheat honey protected DNA and reduced liver damage induced in mice by carbon tetrachloride (Cheng et al., 2015 2015 ). Supplementation with buckwheat honey also resulted in strong 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity and potential ferric reducing antioxidant activity, and enhanced hepatic antioxidant enzymes such as superoxide dismutase and glutathione peroxidase (Cheng et al., 2015 2015 ). Most bioactive compounds undergo extreme gastrointestinal digestion before exerting their biological activity. Some studies have shown an increase in the biological activity of compounds subjected to in vitro digestion (Chen et al., 2016a 2016a ; 2016b 2016b ). Although belonging to the same species, different forms of buckwheat may contain different bioactive compounds with variable potency. Most forms of buckwheat have been extensively studied, but there have been no studies of TBW rice, which is widely used as a functional food in the Yunnan and Sichuan Provinces and other parts of China. Therefore, in this study, we chose to assess the impact of in vitro simulated digestion on the content and antioxidant and antidiabetic properties of flavonoids in TBW rice. Materials and methods 1 Materials and regents 2 TBW rice was purchased from the Kunming Dianqiao Food Co., Ltd. (Yunnan, China). The samples were stored in a cool and dark place. 2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), DPPH, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), rutin, quercitrin α-glucosidase, p -nitrophenol glucopyranoside ( p NPG), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), 2',7'-dichlorofluorescein diacetate (DCFH-DA), pepsin, pancreatin, and bile salts were obtained from Sigma-Aldrich (St. Louis, MO, USA). Vitamin C, bovine serum albumin (BSA), and anthrone were purchased from the Aladdin Reagent Co., Ltd. (Shanghai, China). Glucose assay kits were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other reagents used were of analytical grade. Extraction of flavonoids from TBW rice 2 Two hundred grams of TBW rice was firstly ground to a powder. Then, 5 g of the powder was accurately weighed and extracted with 120 ml of ethanol-water (60%, v/v) at 70 °C for 60 min. The extraction process was repeated twice. The flavonoid extract was centrifuged at 4000 r/min for 10 min. After centrifugation, the supernatant was collected and subjected to evaporation at 40 °C to remove ethanol. The remaining fluid was subjected to lyophilization for 48 h. The lyophilized powder (LP) sample of TBW rice flavonoid extract (TBFE) was stored at −80 °C for further analysis. In vitro digestion 2 To assess the influence of simulated digestion on TBW rice flavonoids and their biological activity, the flavonoid extract was subjected to simulated in vitro digestion as described previously, with slight modification (Chen et al., 2016a 2016a ). Briefly, 100 mg of TBFE was dissolved in 10 ml of distilled water. Porcine pepsin (3000 U/mg, 20 mg) was added to the mixture, which was then adjusted to pH 2.0 with 1 mol/L HCl. Then the sample was incubated at 37 °C in a shaking water bath for 1 h at 100 r/min. The pH was adjusted to 6.0 with 1 mol/L sodium bicarbonate, and then 20 mg of pancreatin and 120 mg of bile salts were added. The mixture was adjusted to pH 7.5 with 1 mol/L sodium hydroxide and incubated at 37 °C in a shaking water bath for 2 h at 100 r/min. After incubation, the digested sample was centrifuged at 8000 r/min for 10 min. The supernatant was collected and made up to a final concentration of 6 mg TBFE/ml by diluting with distilled water. The TBW rice flavonoid extract digest (TBFD) was stored at −80 °C for further investigation. Identification of flavonoids by HPLC 2 Flavonoid compounds of TBW rice (digested and non-digested) were analyzed using a high performance liquid chromatography (HPLC) system (Waters, USA) with a diode array detector. The column was Promosil C18 (4.6 mm×250 mm, 5 μm) operated at 30 °C. The mobile phase consisted of solvent A (water and formic acid, 98.5/1.5, v/v) and solvent B (formic acid, acetonitrile, methanol, and water, 1.5:22.5:22.5:48.5, v/v/v/v). The flow rate was set at 1.0 ml/min and the injection volume was 10 μl. A linear gradient program was applied as follows: 0–35 min from 93% to 75% (A), 35–45 min from 75% to 35% (A), 45–46 min from 35% to 0% (A), 46–50 min 0% (A), 50–57 min from 0% to 93% (A), then 93% (A) for 3 min. The spectra of flavonoid compounds were recorded at 360 nm. Rutin and quercitrin were used as standards. Antioxidant assay 2 2.5.1 DPPH assay The free radical scavenging capacities of TBFE and TBFD were evaluated using the DPPH assay as previously described, with slight modification (Chen et al., 2014a 2014a ). Briefly, 100 μl of TBFE or TBFD was added to 500 μl of a 0.1 mmol/L solution of DPPH in ethanol and incubated in darkness at room temperature for 30 min. After incubation, the absorbance was measured at 517 nm. The percentage inhibition of DPPH radicals caused by TBFE and TBFD was determined according to the following formula: Inhibition rate=[( A control − A sample )/ A control ]×100%, (1) where A control and A sample are the absorbances of the control and sample, respetively. Ascorbic acid was used as an antioxidant standard. 2.5.2 ABTS assay The ABTS radical cation (ABTS •+ ) assay (Floegel et al., 2011 2011 ) was used to evaluate the radical scavenging activities of TBFE and TBFD, with slight modifications. Briefly, ABTS •+ stock solution was prepared by reacting 10 ml of 7 mmol/L 2,2'-azobis (2-amidinopropane) dihydrochloride with 179 ml of 140 mmol/L aqueous potassium persulfate. The mixture was incubated for 12 h at room temperature in darkness. A working solution of ABTS •+ was made by diluting the stock solution 20-fold with phosphate buffer saline (PBS) for further use. Six hundred microliters of ABTS •+ solution were mixed with 20 μl of TBFE or TBFD at different concentrations and, after incubation for 6 min in the dark, the decrease in absorbance at 734 nm was monitored. The ABTS •+ radical scavenging activities of TBFE and TBFD are expressed as mg vitamin C equivalent (VCE)/g LP sample. 2.5.3 FRAP assay The ferric reducing antioxidant power (FRAP) assay (Chen et al., 2013a 2013a ) was used to assess the ferric reducing activities of TBFE and TBFD. Ten milliliter of 10 mmol/L 2,4,6-tripyridyl- S -triazine (TPTZ) solution, 10 ml of 20 mmol/L FeCl 3 , and 100 ml of 300 mmol/L acetate buffer (pH 3.5) were mixed to produce a FRAP working solution. Fifty microliters of TBFE or TBFD were mixed with 500 μl of FRAP solution. The mixture was then incubated at 37 °C for 30 min, and the absorbance was measured at 593 nm. Vitamin C was used as an antioxidant standard. α-Glucosidase inhibitory assay 2 The a-glucosidase inhibitory assay was adapted according to a previously described method (Yilmazer-Musa et al., 2012 2012 ). Briefly, 5 μl of the α-glucosidase solution (5 U/ml, dissolve in 0.1 mol/L potassium phosphate buffer, pH 6.9) was added to 20 μl of sample solution at different concentrations and made up to 500 μl with 0.1 mol/L potassium phosphate buffer (pH 6.9). The reaction mixture was incubated at 37 °C for 20 min. The reaction was then initiated by adding 10 μl of p NPG (10 mmol/L) as substrate and the mixture was incubated at 37 °C for 30 min. Finally, the reaction was terminated by the addition of 500 μl of 1 mol/L Na 2 CO 3 . Absorption was measured at 405 nm, and inhibitory activity was calculated using Eq.(1). Cell culture 2 Human HepG2 cells were obtained from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured as previously described (Chen et al., 2014b 2014b ). Briefly, HepG2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% (v/v) fetal calf serum, 100 U/ml penicillin, and 100 U/ml streptomycin at 37 °C, with 5% CO 2 in a humidified incubator. Cell viability assay 2 Cell viability was determined using the MTT method as previously described (Chen et al., 2013b 2013b ). Briefly, the cells were seeded into 96-well culture plates at a density of 5×10 3 cells/well and incubated for 24 h. After incubation, the cells were treated with TBFE and TBFD at different concentrations for 24 h. The medium was then removed and MTT (0.5 mg/ml) was added to each well and the plates were incubated for 4 h. The formazan precipitate was dissolved by the addition of 150 μl of dimethyl sulfoxide (DMSO) to each well. Absorbance was measured at 490 nm using a Tecan Infinite M200 microplate reader (Molecular Devices, USA). Determination of cellular ROS 2 Cellular ROS contents were determined as previously described, with some modification (Chen et al., 2012a 2012a ). Briefly, HepG2 cells were seeded into 12-well culture plates at a density of 5×10 5 cells/well. After incubation for 24 h, cells were treated with TBFE or TBFD at different concentrations. After treatment for 24 h, cells were incubated with 300 μmol/L H 2 O 2 for a further 6 h. Cells were collected and incubated with 10 μmol/L DCFH-DA at 37 °C for 30 min, then washed with PBS and immediately analyzed using a fluorescence microscope (Nikon, Japan). The results are expressed as mean DCF fluorescence intensity calculated using the image analysis software Image-Pro Plus 6.0 (Media Cybernetics, Inc., USA), from six different microscopic fields. Glucose consumption 2 A glucose consumption assay was used according to a previous method, with some modifications (Lv et al., 2014 2014 ). Briefly, HepG2 cells were seeded into 96-cell culture plates at a concentration of 5×10 3 cells/well. After incubation for 24 h, the cells were washed with PBS twice and the medium was replaced by serum-free DMEM with 0.2% BSA. The medium was then supplemented with TBFE, TBFD, or metformin (2 mmol/L). The glucose concentration in the medium was determined after 24 h incubation by the glucose oxidase method, using a glucose assay kit. Consumption of glucose was first calculated from the difference between the concentrations in the medium at the beginning and after an appropriate time of culture, and then normalized to the results of the MTT assay. Glycogen content 2 Glycogen content was determined as previously described, with slight modification (Ouyang et al., 2011 2011 ; Huang et al., 2015 2015 ). Briefly, HepG2 cells were seeded into 6-well plates at a concentration of 2×10 5 cells/well and then treated with TBFE, TBFD, or metformin (2 mmol/L) for 24 h. Cells were collected and adjusted to the same concentration. The cells were then homogenized using 0.5 ml of 30% (0.3 g/ml) potassium hydroxide and boiled for 15 min to destroy glucose, followed by centrifugation at 15 000 g for 15 min. A volume of 1.5 ml of ethyl alcohol was then added. The glycogen content was determined using anthrone reagent. The absorbance of the color generated by the reaction was detected at 620 nm and the glycogen content values were calculated using glucose as a reference standard. Statistical analysis 2 Data are expressed as mean±standard deviation (SD) from at least three independent experiments. One-way analysis of variance (ANOVA) followed by multiple range significant difference (Duncan) tests ( P < 0.05) was carried out using SPSS 22.0. Results 1 Flavonoid identification by HPLC 2 TBW rice was used for the extraction of flavonoids. After ethanol-water extraction of flavonoids, the flavonoid extract of TBW rice was subjected to simulated in vitro digestion to find the impact of in vitro digestion on flavonoids and their biological activity. The types and concentrations of TBFD and TBFE were determined by HPLC. HPLC analysis showed the presence of rutin in a major proportion, while a small amount of quercitrin was detected. The retention time of rutin and quercitrin was 48.823 and 49.480 min, respectively, in TBFE (Fig. 1a 1a ) and 48.813 and 49.470 min, respectively, in TBFD (Fig. 1b 1b ). A slight decrease in the amount of rutin and quercitrin was found in TBFD compared to TBFE. The amounts of rutin in TBFE and TBFD were 46.42 mg/100 mg LP (LP of TBW rice flavonoid) and 43.42 mg/100 mg LP, respectively, and the amounts of quercitrin in TBFE and TBFD were 6.43 mg/100 mg LP and 5.74 mg/100 mg LP, respectively (Table 1 1 ). LP: lyophilized powder. The values are expressed as mean±SD ( n =3) Content Rutin (mg/100 mg LP) Quercitrin (mg/100 mg LP) TBFE 46.42±0.41 6.43±0.07 TBFD 43.42±0.32 5.74±0.08 Antioxidant activities of TBFE and TBFD 2 After quantitative determination of flavonoid concentrations, antioxidant assays (ABTS, DPPH, and FRAP) were performed to evaluate the antioxidant activities of TBFE and TBFD. The results are presented in Table 2 2 . According to the ABTS assay, the antioxidant activities of TBFE and TBFD showed a concentration-dependent response (Fig. 2a 2a ). The antioxidant activities of TBFE and TBFD (ABTS assay) were 101.87 and 63.57 mg VCE/g LP, respectively. The DPPH radical scavenging activities of TBFE and TBFD were 110.80 and 46.24 mg VCE/g LP, respectively. Both TBFE and TBFD showed a significant free radical scavenging activity against DPPH free radicals in a concentration-dependent response (Fig. 2b 2b ). Furthermore, the antioxidant activities of TBFE and TBFD according to the FRAP assays were 19.37 and 7.00 mg VCE/g LP, respectively. These results indicate that both TBFE and TBFD have a potent antioxidant capacity and also suggest that flavonoids exhibit potential antioxidant activity even after digestion. VCE: vitamin C equivalent; LP: lyophilized powder; IC 50 : 50% inhibition 2 Sample 3 Antioxidant activity (mg VCE/g LP) 2 Inhibition activity of α-glucosidase, IC 50 (mg/ml) ABTS DPPH FRAP TBFE 101.87 110.80 19.37 0.31 TBFD 63.57 46.24 7.00 0.42 Acarbose 0.25 The results were expressed as mean±SD ( n =3) Intracellular ROS scavenging capacities of TBFE and TBFD 2 The cytotoxicities of TBFE and TBFD were evaluated in HepG2 cells using the MTT method, prior to analysis of their ROS scavenging capacities. Treatment of HepG2 cells with TBFE or TBFD for 24 h did not induce any remarkable changes up to 100 μg/ml. However, a decrease in cell viability was observed at 200 μg/ml TBFD (Fig. 3 3 ). Metformin at 2 mmol/L had no cytotoxic effect, indicating that it could be used as a positive control in further experiments. To assess the ROS scavenging activities of TBFE and TBFD, HepG2 cells were pre-treated with TBFE or TBFD (10–100 μg/ml). Then ROS were generated by exposure of cells to H 2 O 2 , a potential ROS generator. To evaluate the level of intracellular ROS, the fluorescence probe DCFH-DA was used. DCFH-DA is a non-fluorescent dye, which can easily penetrate cells and is hydrolysed to DCFH by intracellular esterase. DCFH would be oxidized to DCF by cellular ROS. The ROS scavenging activities of TBFE and TBFD were compared to H 2 O 2 non-stimulated blanks and sample non-treated control groups. TBFE and TBFD at a concentration of 100 μg/ml significantly and equally inhibited ROS production (Fig. 3b 3b ). Therefore, our results indicate that flavonoids of buckwheat rice have appreciable ROS scavenging activity, even after digestion (Fig. 3c 3c ). (a) Cells were treated with TBFE and TBFD at various concentrations and metformin (Met; 2 mmol/L), and cell viability was analyzed by MTT assay. (b) Cells were treated with 300 μmol/L H 2 O 2 in the presence or absence of TBFE or TBFD (10, 50, or 100 μg/ml) for 6 h. Cells were collected and incubated with 10 μmol/L DCFH-DA at 37 °C for 30 min, then washed with PBS and evaluated by fluorescence microscope. (c) The results were expressed as mean DCF fluorescence intensity according to (b). Data are expressed as mean±SD of three independent experiments. Means represented with the same letters are not significantly different according to Duncan test ( P > 0.05) α-glucosidase inhibitory activities of flavonoids in TBFE and TBFD 2 An α-glucosidase inhibitory assay was performed to evaluate the antidiabetic properties of TBFE and TBFD. From our experimental results, we concluded that both TBFE and TBFD showed a significant inhibition of α-glucosidase. Their inhibitory effects were evaluated at different concentrations, and were shown to be concentration-dependent (Fig. 4 4 ). Based on the inhibition of α-glucosidase at various concentrations of TBFD and TBFE, the concentration that provided 50% inhibition (IC50) was obtained for each fraction (Table 2 2 ). The α-glucosidase inhibitory activities of TBFE and TBFD were evaluated using acarbose (a clinical drug) (IC50=0.25 mg/ml) as a positive control. According to our results, TBFE had a comparatively low IC50 value (0.31 mg/ml) compared to TBFD (IC50=0.42 mg/ml). However, the flavonoids, even after digestion, had a tendency to inhibit α-glucosidase. Such inhibition would eventually be expected to represent considerable antidiabetic activity. The results were expressed as mean±SD ( n =3) 3.5 Effects of TBFE and TBFD on glucose consumption in HepG2 cells 2 To assess the effects of TBFE and TBFD on glucose consumption, HepG2 cells were used as a model. Both TBFE and TBFD increased glucose consumption at each concentration compared to controls. However, at 10 μg/ml, the increased glucose consumption was significantly higher (Fig. 5a 5a ). (a) Effect of TBFE, TBFD, and metformin (Met; 2 mmol/L) on glucose consumption after 24 h treatment of HepG2 cells; (b) Changes in the glycogen content of HepG2 cells after 24 h treatment of TBFE and TBFD at various concentrations and Met (2 mmol/L). The results were expressed as mean±SD ( n =3). Means represented with the same letters are not significantly different according to Duncan test ( P > 0.05) Effects of TBFE and TBFD on glycogen content in HepG2 cells 2 The glycogen content was measured in order to understand the reason for the observed increase in glucose consumption in HepG2 cells. Metformin was used as positive control to compare the effects of TBFE and TBFD. Based on the glucose consumption status of the HepG2 cells, two concentrations (10 and 50 μg/ml) were selected to investigate the effects of TBFE and TBFD on glycogen levels. Both TBFE-and TBFD-treated cells showed an increase in glycogen content compared to control cells at both concentrations. The increase was greater than that found in metformin-treated cells (Fig. 5b 5b ). Discussion 1 In this study, we evaluated the presence of flavonoids in TBW rice, which is rather different from other types of buckwheat. Buckwheat is used in diets in various forms such as groats, flour, bran, and buckwheat honey. People from the Tibet Autonomous Region of China and other parts of China use buckwheat in the form of “rice”. The content and composition of the flavonoids of buckwheat vary among buckwheat species and sources such as flowers, leaves, and stems. TBW sprouts are known to show higher antioxidant activities, such as reducing power, free radical scavenging activity, and super oxide scavenging activity, than common buckwheat (Lin et al., 2008 2008 ). Buckwheat sprouts are considered a functional food due to their nutritional benefits and health-promoting activities (Kim et al., 2004 2004 ; Zhang et al., 2015 2015 ). Some studies have suggested that the flavonoid content of buckwheat could be the reason behind its observed biological activity. Increases in the contents of rutin and quercitrin possibly have a role in the enhanced antioxidant capacity of TBW (Lin et al., 2008 2008 ). In other studies, subjecting buckwheat extracts to human in vitro digestion was reported to increase antioxidant capacity via an increase in the contents of the flavonoids rutin and quercitrin (Hur et al., 2011 2011 ). Therefore, in this study, a flavonoid fraction of TBW was subjected to in vitro digestion and analyzed using HPLC to evaluate the impact of in vitro digestion on flavonoids. We found no significant increase in the rutin or quercitrin content of TBW rice after digestion. In fact, we found a slight decrease in rutin and quercitrin levels. This prompted us to look into the biological activity of digested and non-digested flavonoid extracts of TBW rice. Digested and non-digested flavonoid fractions of TBW rice were tested for their radical scavenging capacities by DPPH assay, reduction of radical cations by ABTS assay, and reduction of ferric to ferrous ions by FRAP assay. Our results indicated that the flavonoids showed potent antioxidant activity. Antioxidant activity was reduced, but still potent, after digestion. However, our results contradict those of earlier reports which found in vitro human digested buckwheat (whole grain) flavonoids showed a profound increase in antioxidant capacity (Hur et al., 2011 2011 ). Comparisons of the antioxidant capacity of flavonoids such as rutin, quercitrin, and isoquercitrin of TBW suggested that quercitrin has the strongest antioxidant capacity among these three flavonoids. Flavonoid compounds of buckwheat are known to reduce the intracellular antioxidase activity, thereby increasing cellular oxidative stress. Treatment of hepatoma cells with quercitrin showed the strongest cytotoxic effect due to G2/M phase arrest accompanied by an increase in apoptotic cell death. Therefore, before testing the reactive oxygen scavenging capacities of digested and non-digested TBW rice flavonoids, different concentrations of TBFE and TBFD were tested for their effects on cell viability. In our study, no cytotoxicity was observed following treatment of HepG2 cells with TBW rice flavonoids (digested or non-digested). Our results indicated that digested and non-digested flavonoids showed significant ROS scavenging activity. Supplementation with buckwheat and buckwheat-enriched diets is associated with multiple biological activities, such as hypoglycemic, hypercholesterolemic, anti-cancer, and anti-inflammatory effects. In other studies, germination of buckwheat seeds resulted in increased phenolic components and concomitant antioxidant capacity. Those studies recommended buckwheat as a promising functional food, and buckwheat sprouts were suggested as a new vegetable (Zhang et al., 2015 2015 ; Zhou et al., 2015 2015 ). Oxidative stress is a notorious factor in the development of degenerative and age-related diseases such as diabetes (Lee et al., 2007 2007 ; Hosaka et al., 2011 2011 ; Yu et al., 2015 2015 ), cancer (Lee et al., 2013 2013 ; Zhu, 2016 2016 ), immunity (Yu et al., 2016 2016 ), liver injury (Zhang et al., 2014 2014 ; Pang et al., 2015 2015 ), cardiovascular (Andriantsitohaina et al., 2012 2012 ) and neurodegenerative diseases (Choi et al., 2015 2015 ). Oxidative stress-induced perturbations in the antioxidant defence systems of the body lead to DNA damage and cellular dysfunction, which is further linked to diabetes. One of the strategies adopted to treat diabetes is to find novel bioactive compounds with profound antioxidant capacity. Studies of diabetes and oxidative stress revealed that molecules with potent antioxidant capacity could also inhibit carbohydrate digestive enzymes, such as α-glucosidase and α-amylase. High glucose-induced oxidative stress and insulin resistance were ameliorated by supplementation with TBW flavonoids in hepatic cells via MAPK signalling (Hu et al., 2016 2016 ). The liver plays an important role in metabolism, with numerous functions in the human body including glucose-dependent modulation in glycogen storage, plasma protein synthesis, and detoxification. HepG2 cells are human liver cancer cells, which contain enzymes and metabolic systems similar to those of normal liver cells. HepG2 cells are frequently used as an in vitro model to study cell viability, cellular ROS, glucose consumption, and metabolism. Therefore, in this study, flavonoids of TBW rice in both forms were tested for their effects on α-glucosidase. The flavonoids showed potent α-glucosidase inhibition, even after digestion. Furthermore, supplementation with TBW rice flavonoids resulted in increased glucose consumption and glycogen levels in HepG2 cells. 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Chemical composition and health effects of tartary buckwheat Compliance with ethics guidelines Tao BAO, Ye WANG, Yu-ting LI, Vemana GOWD, Xin-he NIU, Hai-ying YANG, Li-shui CHEN, Wei CHEN, and Chong-de SUN declare that they do not have any conflict of interest. This article does not contain any studies with human or animal subjects performed by any of the authors. 10.1631/jzus.B1600243 Project supported by the Beijing Municipal Science and Technology Plan (No. D151100004015002) and the Research Foundation of the Education Department of Zhejiang Province (No. Y201328143), China 2016-05-30 2016-11-10 2016-09-30 2022-01-05 Copyright © Zhejiang University and Springer-Verlag Berlin Heidelberg 2016 2016 Journal of Zhejiang University-SCIENCE B (Biomedicine and Biotechnology) 12 17