Two-step internal/external polydopamine (Cu²⁺) modified bioceramic scaffolds enhance antibacterial and alveolar bone regeneration capability

Two-step internal/external polydopamine (Cu 2+ ) modified bioceramic scaffolds enhance antibacterial and alveolar bone regeneration capability Xiaojian Jiang 1 first-author Lihong Lei 1 Weilian Sun 1 Yingming Wei 1 Jiayin Han 1 Shuaiqi Zhong 1 Xianyan Yang 2 Zhongru Gou 2 corresp chenlili_1030@zju.edu.cn Lili Chen 1 corresp zhrgou@zju.edu.cn Department of Oral Medicine, the Second Affiliated Hospital, Zhejiang University School of Medicine,Hangzhou,China,310009 Department of Oral Medicine, the Second Affiliated Hospital, Zhejiang University School of Medicine,Hangzhou,China,310009 Bio-nanomaterials and Regenerative Medicine Research Division, Zhejiang-California International Nanosystem Institute, Zhejiang University,Hangzhou,China,310058 Bio-nanomaterials and Regenerative Medicine Research Division, Zhejiang-California International Nanosystem Institute, Zhejiang University,Hangzhou,China,310058 Magnesium-doped calcium silicate (CS) bioceramic scaffolds have unique advantages in mandibular defect repair; however, they lack antibacterial properties to cope with the complex oral microbiome. Herein, for the first time, the CS scaffold was functionally modified with a novel polydopamine (PDA) copper (PDA(Cu 2+ )) rapid deposition method, to construct internal modified (*P), external modified (@PDA) and dual modified (*P@PDA) scaffolds. The morphology, degradation behavior and mechanical properties of the obtained scaffolds were evaluated in vitro. The results showed that the CS*P@PDA had a unique micro-/nano-structural surface and appreciable mechanical resistance. During the prolonged immersion stage, the release of copper ions from the CS*P@PDA scaffolds was rapid in the early stage and exhibited long-term sustained release. The in vitro evaluation revealed that the release behavior of copper ions ascribed an excellent antibacterial effect to the CS*P@PDA, while the scaffolds retained good cytocompatibility with improved osteogenesis and angiogenesis effects. Finally, the PDA(Cu 2+ ) modified scaffolds showed effective early bone regeneration in a critical-size rabbit mandibular defect model. Overall, it was indicated that considerable antibacterial property along with the enhancement of alveolar bone regeneration can be imparted to the scaffold by the two-step PDA(Cu 2+ ) modification, and the convenience and wide applicability of this technique make it a promising strategy to avoid bacterial infections on implants. Polydopamine (Cu2+) modification Antibacterial properties Bone regeneration Angiogenesis Bioceramic scaffolds. 10.1631/jzus.B23Z0004 2023-03-21 2023-04-28 2023-06-10 2023-06-10 38602655