高通量测序在探究中国传统发酵食品菌群多样性及动态变化中的应用

Insights into the microbial diversity and community dynamics of Chinese traditional fermented foods from using high-throughput sequencing approaches 高通量测序在探究中国传统发酵食品菌群多样性及动态变化中的应用 1 2 corresp first-author ‡Corresponding Author ‡Corresponding Author †E-mail:gqhe@zju.edu.cn †E-mail:gqhe@zju.edu.cn gqhe@zju.edu.cn 1 2 1 2 1 2 1 2 College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China Zhejiang Provincial Key Laboratory of Food Microbiology, Zhejiang University, Hangzhou 310058, China Zhejiang Provincial Key Laboratory of Food Microbiology, Zhejiang University, Hangzhou 310058, China Chinese traditional fermented foods have a very long history dating back thousands of years and have become an indispensable part of Chinese dietary culture. A plethora of research has been conducted to unravel the composition and dynamics of microbial consortia associated with Chinese traditional fermented foods using culture-dependent as well as culture-independent methods, like different high-throughput sequencing (HTS) techniques. These HTS techniques enable us to understand the relationship between a food product and its microbes to a greater extent than ever before. Considering the importance of Chinese traditional fermented products, the objective of this paper is to review the diversity and dynamics of microbiota in Chinese traditional fermented foods revealed by HTS approaches. 中国传统发酵食品有着上千年的历史，已经成为中国饮食文化中不可缺少的部分。微生物是发酵食品的灵魂，为了探究传统发酵食品中的微生物组成，多种依赖培养和非培养技术都已应用于不同发酵体系的微生物菌相分析中。高通量测序技术是近几年兴起的生物学技术，它极大地方便了环境微生物多样性的研究，促进了我们对复杂微生物环境的认知，其在发酵食品中的应用提升了我们对发酵食品品质与微生物关系的认识水平。本文综述了高通量测序技术在探究我国传统发酵食品菌相中的应用，总结分析了不同发酵体系中微生物的组成和动态变化。 Chinese traditional fermented foods Microbiota High-throughput sequencing 传统发酵食品 微生物菌群 高通量测序 Introduction 1 Chinese traditional fermented foods have a long history dating back thousands of years (Liu et al., 2011 2011 ). Many kinds of fermented foods have appeared and enjoyed great popularity in China since ancient times. A range of raw materials including grains, vegetables, milk, meat, tea, and beans has been used for fermentation, resulting in a myriad of fermented products like Chinese liquor, traditional aged vinegar, Chinese suancai, and soy sauce. Microbial fermentation not only ensures increased shelf life but also confers other beneficial properties on food in terms of texture, flavor, and nutrition. In other words, food fermentation is not only an effective method for food preservation, but also an economical method for food processing. It is well known that biochemical conversions occurring during the process of fermentation result from the metabolic activities of microorganisms existing in different ecological niches. Thus, microorganisms, the soul of fermented foods, play a crucial role in determining the overall quality attributes of any fermented food. Therefore, unraveling complex microbial communities of fermented foods and understanding their specific effects on the quality of traditional fermented foods is a topic of continuous attention in food microbiology. Selected strains isolated from traditional fermented foods can be used to standardize their production while maintaining their traditional traits. Extensive research has been conducted to uncover the microbial composition of all kinds of Chinese traditional fermented foods and a large variety of microorganisms including archaea, bacteria, and fungi has been revealed. In the past, culture-based methods were the only way to conduct microbiological analysis. These methods are extremely useful in determining the microbial community structure in environments influenced by natural as well as anthropogenic effects. However, they have certain limitations. For instance, many bacterial species lose their ability to grow and switch into a distinct life phase known as the “viable but non-culturable (VBNC)” state, in which they are viable but no longer detectable on the routine bacteriological media on which they would normally grow (Oliver, 2005 2005 ). In the VBNC state, the metabolic activities and growth rates of bacteria slow down and this is why many of them cannot be detected using culturing methods (Fakruddin et al., 2013 2013 ). Bacteria stochastically change into the VBNC state as a result of many environmental cues like starvation, stressful pH, temperature shock, oxygen imbalance, and non-optimal salinity (Du et al., 2007 2007 ; Ayrapetyan et al., 2015 2015 ). Also, some bacteria present in normal and metabolically active form in food or environmental samples do not grow on nutrient-rich agar plates under laboratory conditions. It has been said that microorganisms recovered from the environment by traditional culturing methods are rarely abundant in terms of their number or function in the environment from which they were recovered. Less than 1% of microorganisms are readily culturable and these are considered the weeds of the microbial world (Hugenholtz, 2002 2002 ). The first evidence of the non-culturable ability of bacteria came from the observation that the number of bacterial cells observed under the microscope did not match the number of colonies that formed on agar plates (Winterberg, 1898 1898 ). This plate count anomaly was confirmed by Amann ( 1911 1911 ). It is estimated that only 30 phyla out of the 100 predicted from phylogenetic analysis contain culturable representative microbes (Alain and Querellou, 2009 2009 ). In this context it is important to mention that the terms “uncultured”, “uncultivable”, and “unculturable” have been used in the literature to describe microorganisms that are not readily cultured under laboratory conditions. These terms are misnomers because upon successful cultivation using novel techniques, these previously unculturable organisms become culturable (Lewis et al., 2010 2010 ). One of the strategies for incubating “uncultured” organisms is to use a diffusion chamber. This tricks bacteria into perceiving that they are growing in their natural environment (Kaeberlein et al., 2002 2002 ). So, the use of the word “unculturable” signifies the fact that our current knowledge of their biology and fastidious growth requirements (pH, nutrients, temperature, osmotic conditions, and many more) is limited. However, efforts toward tailoring synthetic growth media to the imaginable natural growth environment of these bacteria have resulted in great success, making thousands of previously “unculturable” bacteria culturable (Stewart, 2012 2012 ). Nevertheless, it remains a challenge to bring these fastidious organisms into the laboratory due to a lack of optimal growth conditions on nutrient-rich laboratory media. This deficiency is probably due to a lack of interest and desire in designing and optimizing new growth media, and the availability of fewer trained laboratory personnel with expertise in microbial physiology and growth conditions (Prakash et al., 2013 2013 ). Based on the above discussion, we conclude that culture-dependent approaches can underestimate the complexity of microbial ecosystems because of bias in selecting the microbes. In contrast, culture-independent approaches, mostly targeting total DNA or RNA rather than relying on traditional cultivation, can provide us with an innovative and comprehensive insight into various microecosystems, including food fermentation systems. During the last two decades, several approaches based on the direct analysis of ribosomal RNA genes have been developed to study directly the genome of environmental microorganisms. These approaches include mainly denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE) (Muyzer, 1999 1999 ), single strand conformation polymorphism (SSCP) (Hayashi, 1991 1991 ), and terminal restriction fragment length polymorphism (T-RFLP) (Marsh, 1999 1999 ). Most recently, high-throughput sequencing (HTS) or next-generation sequencing (NGS) approaches (e.g. pyrosequencing) have drawn much attention because of their power for analyzing whole environmental microorganisms, and have been successfully applied to the elucidation of the microbiota of various fermented foods. This approach enables us to understand the relationship between a food product and its microbes to a greater extent than ever before. In the field of food fermentation, HTS has been widely employed in profiling microbial ecosystems throughout the world (Bokulich and Mills, 2012 2012 ; Kergourlay et al., 2015 2015 ). Considering the extensive and promising applications of HTS, this review will focus on the use of HTS methods and their roles in unraveling the complex microbial ecosystems of Chinese traditional fermented foods. High-throughput sequencing and its application to fermented foods 1 HTS or NGS technology is a revolutionary innovation in the field of gene sequencing and has substantially widened the scope of microbial analysis of environmentally derived samples (Mardis, 2008 2008 ). It is characterized by a high-throughput data yield from a single run. HTS or NGS is the catch-all term used to describe a number of modern sequencing techniques including Roche/454 technology, Illumina (Solexa) technology, ABI SOLiD technology, Ion torrent: Proton/PGM sequencing and PacBio SMRT (Mayo et al., 2014 2014 ). It has provided new platforms to investigate all kinds of microbial communities and to discover some rare and even unknown species. HTS can generate thousands or even millions of sequences at the same time. These sequences help in identifying rarely culturable or unculturable microbes in a sample. HTS also gives an accurate inventory of all microbial operons and genes that are present or are expressed under certain study conditions (Mayo et al., 2014 2014 ). Compared to DGGE, HTS can provide more detailed information with digitalized results (Ling et al., 2010 2010 ). In the past few years, its application has promptly expanded and recent advances in HTS are revolutionizing the study of complex food microbial communities. A great deal of attention has been given to employing these techniques in a variety of food fermentation ecosystems to investigate the composition, structure, and dynamics of microbial populations, and to discover novel, low-abundant, and taxonomic lineages. Based on this knowledge, we can obtain a comprehensive understanding of the relationship between complex microbial communities and the quality of resulting products. Profiling the microbial diversity of fermented foods 2 The advent of HTS has revolutionized the era of biotechnology by transforming scientific enterprise and allowing the possibility of studying minor details of DNA/RNA that have yet remained beyond our imagination. As a result of recent advances in molecular biology, microbial genomics has changed tremendously and is constantly expanding and deciphering more insights into the uncharted microbial life of tiny creatures. It has been estimated that less than 1% of microbes have yet been cultured. Have we really conquered the microbial world or is it just an illusion The discovery of metagenomics has made this question obsolete. The first metagenomic studies on microbiota of environmental samples pointed out that 80% of the bacteria identified by metagenomics or by 16S ribosomal RNA (rRNA) pyrosequencing genes had not yet been cultured (Venter et al., 2004 2004 ). HTS has gained much attention in the field of food microbiology and has provided a myriad of novel technologies to detect and monitor microbes in food, allowing the possibility of determining the molecular mechanisms of food functionality. It is well established that the whole microbial profile cannot be determined using culture-dependent methods (Riesenfeld et al., 2004 2004 ) as they provide a biased picture of overall microbial communities. However, the use of metatranscriptomic approaches, involving the extraction of DNA/RNA directly from a fermented food matrix, has overcome this limitation. In short, DNA or complementary DNA (cDNA, from reverse transcription of RNA) is subjected to polymerase chain reaction (PCR) for the amplification usually of 16S rRNA genes. The use of 16S metagenetic analysis has resulted in a novel description of bacterial consortia associated with cheeses, in which several new bacterial genera, like Prevotella and Arthrobacter , were reported for the first time (Quigley et al., 2012 2012 ). Metagenomic analyses of various fermented foods like sourdough (Liu et al., 2016 2016 ), kefir (Marsh et al., 2013 2013 ; Nalbantoglu et al., 2014 2014 ), fermented mung beans (Chao et al., 2013 2013 ), Mexican Cotija cheese (Escobar-Zepeda et al., 2016 2016 ), and kimchi (Park et al., 2011 2011 ) have been performed. A recently published review provides detailed information on the application of high-throughput 16S rRNA sequencing to a variety of fermented foods to elucidate microbiota (Kergourlay et al., 2015 2015 ). Investigation of the microbial dynamics of fermented foods 2 Strain monitoring is one of the major applications of HTS in food microbiology. Strain monitoring helps to determine fermentation dynamics, knowledge of which is very important for the proper microbiological risk management of fermented foods. Although most fermented foods are considered safe, some incidents of foodborne infection have been attributed to the consumption of fermented foods (Shaffer et al., 1990 1990 ; Centers for Disease Control and Prevention, 2001 2001 ). Therefore, monitoring temporal microbial succession, the fate of starter cultures and initial foodborne pathogens during the fermentation process and the final residing microbes of any fermented food is of crucial importance. The food safety of some spontaneously fermented foods can be questioned because of the presence of pathogens in the initial food matrix. HTS has successfully been used to determine the population dynamics of microbial consortia in a variety of fermented foods, including bacterial community temporal succession during the fermentation process of wine grapes (Piao et al., 2015 2015 ), sourdoughs (Weckx et al., 2010 2010 ; Ercolini et al., 2013 2013 ), Mongolian fermented cow’s milk (Liu W. et al., 2015 2015 ), mozzarella cheese (Ercolini et al., 2012 2012 ), and kimchi (Jung et al., 2011 2011 ). Revealing microbial activities of fermented foods 2 In the complex microbial diversity of fermented foods, there are a number of technically important microbial strains which impart useful properties to food, like aroma and flavor (Smit et al., 2005 2005 ). It is well known that the performance of a microbe during fermentation can be understood by its gene expression. Metatranscriptomic approaches (the use of messenger and non-coding RNAs) provide important insights into regulatory networks and gene expression during the sampling stage, and in combination with the metaproteome and metabolome, the metatranscriptome provides information concerning the activities and functions of microbial communities (Abram, 2015 2015 ). Using metatranscriptomic analysis, the global gene expression of microbes during the fermentation process can be determined, which helps to understand the key metabolic activities of microbes and to assess the safety of the process. For instance, the gene expression of lactic acid bacteria (LAB) during the fermentation of kimchi (Jung et al., 2013 2013 ) and sourdough (Weckx et al., 2011 2011 ) was determined using this technique, which helped to determine the functional role of each microbe based on the expression of genes. Application of high-throughput sequencing in Chinese traditional fermented foods 1 Chinese traditional fermented foods are produced mostly using unique procedures involving complex microbial communities, the profiling of which by traditional culture methods is a great challenge. Food fermentation is a dynamic process involving various endogenous and exogenous factors such as temperature, humidity, and acidity, and as a result, the microbiota involved undergo dynamic changes, which in turn may exert their influence on the fermentation process. Therefore, revealing the dynamics of microbiota is necessary to find out the relationship between microorganisms and the quality and safety of food. Since most of the microbes are difficult to cultivate, traditional culturing remains unsuccessful in determining the microbial community dynamics of any fermented food. However, HTS, based on its powerful ability to decipher complex microbial communities, has gradually been applied by researchers to elucidate the composition and dynamics of microbiota in different kinds of Chinese traditional fermented foods (Table 1 1 ) and has already offered unprecedented insights. 2 Product or fermentation system 2 Sequencing method or platform 2 Target gene(s) 2 Reference Bacteria Fungi Daqu for light-flavor liquor Pyrosequencing V1–V3 (16S rRNA) Zhang et al., 2014 2014 Starter of Fen liquor Pyrosequencing ITS1 Li et al., 2013 2013 Fermented grains of Fen liquor Pyrosequencing ITS1 Li et al., 2011 2011 Cellar mud for strong aromatic liquors Pyrosequencing V3–V5 (16S rRNA) Wang C.D. et al., 2014 2014 Cellar mud for strong aromatic liquors Illumina MiSeq V4 (16S rRNA) Zheng et al., 2015 2015 Sourdough Illumina HiSeq 2000 V6 (16S rRNA) Zhang and He, 2013 2013 Sourdough Pyrosequencing V3–V5 (16S rRNA) Liu et al., 2016 2016 Pu-erh tea Pyrosequencing Whole genome Whole genome Lyu et al., 2013 2013 Pu-erh tea Pyrosequencing V1–V3 (16S rRNA) ITS Zhao et al., 2015 2015 Zhenjiang aromatic vinegar Pyrosequencing V1–V3 (16S rRNA) Wang et al., 2015 2015 Tianjin Duliu aged vinegar Illumina V3 (16S rRNA) Nie et al., 2013 2013 Tianjin Duliu aged vinegar Pyrosequencing V3–V6 (16S rRNA) Peng et al., 2015 2015 Shanxi aged vinegar Illumina MiSeq V4 (16S rRNA) V4 (18S rRNA) Li et al., 2015 2015 Shaoxing rice wine Pyrosequencing V3–V5 (16S rRNA) Fang et al., 2015 2015 Shaoxing rice wine Illumina MiSeq V4 (16S rRNA) Liu S.P. et al., 2015 2015 Shaoxing rice wine Pyrosequencing V3 (16S rRNA) Wang P. et al., 2014 2014 Shaoxing rice wine Illumina HiSeq 2000 Whole genome Xie et al., 2013 2013 Yoghurt of Xinjiang Pyrosequencing V1–V3 (16S rRNA) V4 (18S rRNA) Xu et al., 2015 2015 Tarag Pyrosequencing V3 (16S rRNA) ITS1 Sun et al., 2014 2014 Tibetan kefir Illumina HiSeq 2000 V6 (16S rRNA) Gao et al., 2013 2013 Chinese liquors 2 In Chinese history, alcohol has been termed the “water of history” because of its role in folklore. The use of alcoholic fermented products has great significance in the dietary culture of China. Among these products, Chinese liquor enjoys unique status in the cultural life of Chinese people. Chinese liquor is one of the six most famous distilled spirits throughout the world. There are many varieties of Chinese liquor based on different production techniques and distinct liquor starters. It is usually produced with the use of a unique starter culture called Daqu, a saccharifying and fermenting agent. Daqu is usually prepared by solid state fermentation from wheat, barley, and/or peas, usually in the form of cakes or bricks. The bricks are subjected to a long period of incubation during which large amounts of enzymes are produced and high microbial loads, including bacteria, yeasts, and molds, develop. Daqu preparation is manipulated under various specific time-temperature control schemes to obtain liquors with distinctive flavors. The most famous flavor types include sauce-flavor (e.g. Maotai liquor), strong-flavor (e.g. Luzhou liquor), light-flavor (e.g. Fen liquor), rice-flavor (e.g. Sanhua liquor), and feng-flavor (e.g. Xifeng liquor). The process generally involves different starters, various fermenting techniques, and distinct geographical environments. Together, these result in different microbial successions during fermentation leading to the production of liquors with distinct flavors. Wang C.D. et al. ( 2014 2014 ) compared the microbiota of a 10-year-old cellar and a 1-year-old cellar used for the production of Luzhou-flavor liquors using a pyrosequencing approach and concluded that long-term spontaneous batch-fermentation accumulates diverse adaptive microbes belonging to the genera Aneurinibacillus , Bacillus , Clostridium , and Lactobacillus , which together produce more flavorful liquors. HTS also showed the prevalence of Lactobacillus and Clostridium in both 30-and 300-year-old pit muds of Chinese Luzhou-flavor liquors (Zheng et al., 2015 2015 ). On the other hand, Wickerhamomyces , Kluyveromyces , and Pichia were the main fungal genera and there was no obvious discrimination within the fungal community in pit muds of different ages. Besides the Luzhou-flavor liquors, the variability of bacteria and fungi present during the fermentation process of Fen liquor was also investigated by HTS. Zhang et al. ( 2014 2014 ) analyzed the bacterial community of three kinds of Daqu used for Fen liquor brewing using pyrosequencing. Most of the annotated reads were assigned to two phyla, namely Firmicutes and Actinobacteria, and Firmicutes was the most abundant group. LAB were present as an important group in all three samples, accounting for 45.8%, 46.3%, and 24.7% of the bacteria, respectively. Investigation of fungal diversity showed the presence of six families and one genus, i.e. Saccharomycetaceae, Saccharomycopsidaceae, Saccharomycodaceae, Dipodascaceae, Trichocomaceae, Pleosporaceae, and Candida , with little variation in composition among different samples (Li et al., 2011 2011 ; 2013 2013 ). We conclude from all the reports that LAB have a significant influence on the fermentation of different liquors. Chinese sourdough 2 Sourdough is a mixture of flour and water that is fermented by yeast and LAB. It has played a significant role in making flour-based fermented food for a very long time all around the world (Hammes and Gänzle, 1997 1997 ). It is widely used as a starter for bread making throughout Europe (de Vuyst et al., 2002 2002 ; Ktenioudaki et al., 2015 2015 ) and likewise, is employed as an ideal inoculum in making Chinese steamed bread (Li et al., 2014 2014 ; Zhang et al., 2016 2016 ). Sourdough fermentation significantly improves the quality of the resultant product, in terms of texture, flavor, and nutrition, and prolongs the shelf life by retarding the staling process of starch and decelerating microbial spoilage. Extensive research effort has been directed toward the investigation of the microbiota involved in sourdough fermentation in different European countries using culture-dependent and culture-independent approaches (de Vuyst et al., 2014 2014 ). Generally, both approaches obtained similar outcomes, but culture-independent approaches, especially HTS, often provided more information, like the situation in other fermented foods (Elizaquivel et al., 2015 2015 ). The results showed that yeasts and LAB dominated this specific niche, and Lactobacillus sanfranciscensis is regarded as the key sourdough LAB species (Gobbetti and Corsetti, 1997 1997 ). However, in the case of Chinese traditional sourdough, which has been widely used and distributed around China, there remains a relative lack of knowledge of the microbial composition. Zhang and He ( 2013 2013 ) investigated the major bacteria in five sourdough samples collected from different places using the Illumina HiSeq 2000 system and found that Lactobacillus , Leuconostoc , and Weissella were the predominant genera among the five samples. Zhang et al. ( 2011 2011 ) investigated the diversity of LAB and yeasts in traditional sourdoughs collected from the western region of Inner Mongolia Autonomous Region in China by culturing and PCR-DGGE at the species level. The results showed that Lactobacillus plantarum group and Saccharomyces cerevisiae were the predominant microflora in their samples. Nevertheless, studies performed by other researchers using pyrosequencing revealed that the dominant LAB species in Chinese sourdough was L. sanfranciscensis (Liu et al., 2016 2016 ). This bacterium proved to be the only species present among all fifteen sourdoughs and it accounted for more than half of the bacteria in 9 of the 15 samples. The dominance of L. sanfranciscensis in Chinese sourdoughs has also been confirmed by culture-dependent and DGGE approaches (Zhang et al., 2015 2015 ). These conflicting results may be attributed to the different sampling regions and culture conditions used. L. sanfranciscensis is fastidious (Kline and Sugihara, 1971 1971 ), and the conditions applied by Zhang et al. ( 2011 2011 ) were not appropriate for its recovery. HTS, however, can effectively avoid the bias caused by culturing approaches. Furthermore, pyrosequencing revealed that more than 90% of the bacteria belonged to LAB in 14 of the 15 sourdough samples analyzed. A total of 24 LAB species were found by pyrosequencing, 17 of which were lactobacilli (Liu et al., 2016 2016 ). Pu-erh tea 2 Pu-erh or Pu’er is a fermented dark tea, black to brown in color, with a moderate taste, and is produced only in Yunnan Province, China. The role of Pu-erh tea as an anti-oxidative agent for reducing cholesterol levels and aiding digestion is well acknowledged (Hou et al., 2009 2009 ; Cao et al., 2011 2011 ). There are two main categories of Pu-erh tea, namely raw and ripened (Tian et al., 2013 2013 ). The processing of raw Pu-erh tea includes the fixation, rolling, and drying of leaves before the tea is compressed into bricks, cakes, or other shapes. The processing of ripened tea involves an extra step called wet piling which is similar to composting and is performed before drying to facilitate the aging process. This step involves fermentation of the leaves in a warm and humid environment by bacterial and fungal cultures. The special flavor and characteristics of this tea result from complex microbial interactions and natural oxidation. Microbiota has a significant influence on the quality of Pu-erh tea, especially during the wet piling. Various kinds of bio-techniques including DGGE (Tian et al., 2013 2013 ; Yang et al., 2013 2013 ), pyrosequencing (Lyu et al., 2013 2013 ), metatranscriptomics (Jiang et al., 2012 2012 ), metagenomics and metaproteomics (Zhao et al., 2015 2015 ) have been used to investigate the microbial composition during the piling process. However, the microbiological profiles of Pu-erh tea are not well understood. There is no doubt that bacteria and fungi cooperate in the piling fermentation, but no consensus on the microbial composition or on which group plays the dominant role in the fermentation process has been reached (Jiang et al., 2012 2012 ; Lyu et al., 2013 2013 ). An integrated metagenomics and metaproteomics investigation of the microbial communities and enzymes in fermentation of Pu-erh tea demonstrated that the dominant bacteria and fungi were Proteobacteria (48.42%) and Aspergillus (94.98%), respectively, and that Aspergillus was the major host of identified proteins (50.45%) (Zhao et al., 2015 2015 ). Similarly, Jiang et al. ( 2012 2012 ) compared microbial communities from two different stages during the pile-fermentation of Pu-erh tea using metatranscriptomic analysis and found that Aspergillus niger was the overwhelmingly predominant species at both stages. In contrast, a preliminary metagenomic study of Pu-erh tea during pile fermentation by Lyu et al. ( 2013 2013 ) showed a significantly lower percentage of eukaryota by metagenomic analysis, indicating the dominant role of bacteria in the whole piling fermentation process. Furthermore, yeasts rather than molds accounted for the overwhelming majority of eukaryota. These discrepancies may result from different Pu-erh tea materials and/or different production conditions, or sequencing depths (Lyu et al., 2013 2013 ). In terms of bacterial composition, results vary among different research studies and differences are found when different methods are used. Further research is required to elucidate the microbial successions occurring during the fermentation of Pu-erh tea and to determine the relationship between the microbes and the quality characteristics of the tea. Chinese vinegar 2 Chinese traditional vinegar (CTV) or Cu, also known as cereal vinegar, is not only an important seasoning for Chinese dishes but also a significant element in Chinese daily diet. The production of vinegars in China can date back 3000 years (Shi, 1999 1999 ). Chinese vinegars are sweeter than western white or cider vinegars. Their average acidity is about 5%–6% with some residues of sugar remaining after fermentation. There is a large variety of Chinese vinegars (including black, white, red, smoky, and herbal) which are categorized based on major or subtle differences in the raw materials, manufacturing process or the nature of the saccharifying agent. Most CTVs are brewed with starchy cereals such as rice, wheat, millet, sorghum, or a combination thereof, by solid-state fermentation that consists mainly of the following stages: preparation of a starter Daqu (if needed, e.g. Shanxi aged vinegar), starch saccharification (SS), alcoholic fermentation (AF), and acetic acid fermentation (AAF). Also, a period of time for aging is often indispensable. Unlike the pure-culture liquid fermentation process of vinegar production in European countries (Tesfaye et al., 2002 2002 ), the fermentation of CTV involves various microbes and complicated but regular successions. Zhenjiang aromatic vinegar, one of the most famous traditional vinegars in China, has a comparatively stable microbial community with a strict series of changes during the fermentation process. Its unique cycle-inoculation style means that the fermentation culture of the 7th day is used as a starter for the next batch inoculation (Xu et al., 2011 2011 ). Results of pyrosequencing showed that during the first three days of the fermentation of Zhenjiang aromatic vinegar, the number of operational taxonomic units (OTUs) dramatically decreased, indicating the elimination of microorganisms intolerant of a stressful environment. The dynamics of Acetobacter and Lactobacillus in different batches show obvious batch-to-batch uniformity. The relative abundance of Lactobacillus shows an initial dramatic increase followed by a gradual decrease, while the relative abundance of Acetobacter keeps rising throughout the whole fermentation process. Thus, there is a trend of succession from Lactobacillus -dominated fermentation to Acetobacter -dominated fermentation. This succession could be attributable to several environmental stresses: (1) the high level of ethanol in the system supporting the growth of ethanol-resistant acetic acid bacteria; (2) high levels of acetic acid and lactic acid (some species of acetic acid bacteria remain metabolically active in high concentrations of acetic acid); (3) elevated temperature (40–46 °C) selects thermo-tolerant acetic acid bacteria (Wang et al., 2015 2015 ). Another two kinds of typical traditional vinegars in China are Shanxi aged vinegar and Tianjin Duliu aged vinegar, which are distinguished by the use of the starter Daqu, akin to the starter used in Chinese liquor fermentation. Daqu provides multitudinous microorganisms and enzymes for the production of vinegar. In the case of Shanxi aged vinegar, the bacterial and fungal dynamics during the 30-d fermentation processes of Daqu have been investigated by Illumina MiSeq sequencing analysis (Li et al., 2015 2015 ). For bacteria, the results demonstrated that Enterobacteriales and Lactobacillales dominated the whole fermentation process, with their relative abundance dramatically increasing from 7.71% to 62.08% and decreasing from 63.1% to 6.2%, respectively. Streptophyta (Cyanobacteria) accounted for 11.23% initially and then were retrieved at low frequencies ( < 2% of total sequences) until the end of fermentation. Bacillales were found in low abundance from Days 1 to 19, and then clearly increased at the end of fermentation. All these changes were associated with environmental conditions, such as temperature and moisture. During the fermentation, although the temperature reached 53–60 °C and the moisture content declined to 12%, the relative abundance of Enterobacteriales significantly increased due to their remarkable capacity to adapt to a large span of temperatures and moisture levels (Wei et al., 2013 2013 ). Thermophilic Bacillales can also better survive under harsh conditions with the secretion of various degradative enzymes (Simonen and Palva, 1993 1993 ). In contrast, Lactobacillales sharply decreased with increasing temperature and remained stable until the end of the fermentation process, and the same was true for Streptophyta (Li et al., 2015 2015 ). The main genera involved in the whole fermentation process include Erwinia , Lactobacillus , Weissella , Leuconostoc , Pediococcus , Bacillus , Staphylococcus , Methylotenera , Kaistobacter , and Acinetobacter . The fungal communities were less complex than the bacterial communities, with Saccharomycetales, Eurotiales, and Mucorales comprising > 99% of all the sequences. Saccharomycetales and Eurotiales dominated the whole fermentation process, while Mucorales accounted for only about 0.1% on the first three days, increasing significantly to 69.22% on Day 7, and dominating until the end of fermentation. Clearly, the fungal communities also change in relation to environmental conditions (Li et al., 2015 2015 ). The genera involved include mainly Pichia , Rasamsonia , Amylomyces , Saccharomycopsis , Saccharomyces , Rhizomucor , Rhizopus , Lichtheimia , Wickerhamomyces , and Candida . Taken as a whole, the effects of a high incubation temperature and a low moisture level are selective in inducing a shift of microbial communities during the fermentation of Daqu. Tianjin Duliu aged vinegar is made from sticky rice and red sorghum, which are mixed with Daqu for saccharification followed by the addition of water to allow AF. The whole mixture then goes through AAF. The unique feature of this fermentation (a spontaneous process over 30 d) is the exchange of the upper and lower layers of the mixture in an empty urn on the 16th day. Nie et al. ( 2013 2013 ) found that four genera namely Lactobacillus , Nostoc , Acetobacter , and Gluconacetobacter , whose total abundances were about 95%, showed distinct population dynamics using Illumina sequencing. During the whole AAF process, LAB were much more abundant than other bacteria, with Lactobacillus being the major genus. The relative abundances of Acetobacter , Gluconacetobacter , and Nostoc increased as the fermentation progressed. The unique process exerted a special influence on the dynamics of the microbial communities involved. For example, Acetobacter increased from 1.30% on the first day to 14.88% on the fifth day. This shows an apparent association between the abundance of this genus and the accumulation of total acids. The population of this genus decreased to 4.28% on the 16th day of fermentation following the exchange of the upper and lower layers. Interestingly, Nostoc , Nocardioides , Propionibacterium , Pediococcus , and Klebsiella were first found in Chinese traditional vinegars following the use of HTS, indicating that HTS is more efficient in exploring bacterial communities. Peng et al. ( 2015 2015 ) analyzed the AAF process by pyrosequencing and the results showed that microbial communities in all samples were dominated by Firmicutes and Proteobacteria. At the genus level, 37 different genera were identified by OTU classification, 9 of which were found to be shared across samples, including Acetobacter , Buttiauxella , Lactobacillus , Pseudomonas , Sphingomonas , Swaminathania , Bacillus , Serratia , and Gluconacetobacter , which may indicate that these were the stable components during AAF. Shaoxing rice wine 2 Shaoxing rice wine, named after the city Shaoxing, has a unique flavor and high nutritional value. It is quite popular among Chinese people and was first recorded more than two thousand years ago. It is an excellent representative of Chinese traditional wines and is internationally well known. Shaoxing rice wine is distinguished by its unique flavor and taste, largely ascribed to its specific microbiota imparted by the starter wheat Qu. Like Daqu used in the fermentation process of Chinese liquor and traditional vinegar, wheat Qu is responsible for the various enzymes and microbes needed during the fermentation process of Shaoxing rice wine. Almost all studies of the microbiota involved in Shaoxing rice wine fermentation have focused on bacteria. Xie et al. ( 2013 2013 ), employing the Illumina HiSeq 2000 approach, showed a more diverse bacterial composition in a mature wheat Qu than that revealed by the DGGE method. The Qu contained mainly three phyla, namely Actinobacteria, Firmicutes, and Proteobacteria. At the genus level, Staphylococcus , Bacillus , Saccharopolyspora , and Pantoea were the most abundant genera, while Lactobacillus occurred with a very low relative abundance. Wang P. et al. ( 2014 2014 ) investigated changes in bacterial diversity during the fermentation of Chinese rice wines using pyrosequencing and the results showed that the bacterial community structures and diversity varied significantly at different fermentation stages. More than ten genera of bacteria were detected in fermentation broth including Staphylococcus , Lactobacillus , Pseudomonas , Bacillus , Saccharopolyspora , Weissella , and a number of uncultured bacteria. Lactobacillus , which usually cannot be detected in wheat Qu by DGGE analysis (Guan et al., 2012 2012 ; Zhang et al., 2012 2012 ), but can be found in small amounts by HTS, increases throughout the whole fermentation process eventually becoming the dominant bacterial group (Wang P. et al., 2014 2014 ). Saccharopolyspora from wheat Qu has a relatively high abundance with little fluctuation during the process. Bacillus also remains relatively stable, albeit at low abundance. Most other genera undergo an initial flourishing stage followed by a continuous diminution, presumably because of their intolerance to the acid environment attributable to LAB. Liu S.P. et al. ( 2015 2015 ) analyzed bacterial succession during rice wine fermentation using the Illumina MiSeq approach and found that the microorganisms in the fermentation broth belonged to three bacterial phyla, namely Firmicutes, Actinobacteria, and Proteobacteria, with Firmicutes being the dominant phylum. At the genus level, ten dominant genera including Saccharopolyspora , Bacillus , Staphylococcus , Thermoactinomyces , Lactobacillus , Leuconostoc , Lactococcus , Weissella , Pseudomonas , and Enterobacter were identified. Bacillus , Staphylococcus , and Thermoactinomyces increased gradually during primary fermentation, and then decreased slightly post fermentation. The first two genera are aerobic and grew well initially due to the high oxygen supply and low alcohol content. However, post fermentation, the depletion of oxygen and increase in alcohol content prevented their growth. Thermoactinomyces decreased dramatically due to the low temperature post fermentation. LAB had a different tendency. Lactobacillus , Leuconostoc , Lactococcus , and Weissella decreased sharply at first, then increased and eventually dominated the population by the end of the fermentation. Most of the LAB belonged to facultative anaerobic and acid-tolerant groups. These features helped them to grow better and dominate during the post fermentation stage, which is characterized by low oxygen content and low pH (Liu S.P. et al., 2015 2015 ). Fermented dairy products 2 Fermented dairy products have been consumed in China for thousands of years, especially by nomads. Milk from mammals such as cows, horses, ewes, goats, camels, and yaks can be used to ferment, leading to various fermented dairy products throughout China, e.g. Tibetan kefir, tarag, yoghurt, kumiss, and cheese. The fermentation process increases the shelf-life of the products, while enhancing the taste and improving the digestibility of milk. These home-made products are fermented spontaneously by natural starter cultures from previous batches having complex microbial compositions (Hu et al., 2015 2015 ; Azat et al., 2016 2016 ). Understanding the microbial diversity and dynamics of these starters is important for the standardization of Chinese traditional fermented milk products. Gao et al. ( 2013 2013 ) investigated the bacterial diversity in four Tibetan kefir grains from different areas in China using Illumina sequencing. Eleven genera were found: Lactococcus , Lactobacillus , Acetobacter , Shewanella , Leuconostoc , Pseudomonas , Streptococcus , Acinetobacter , Pelomonas , Dysgonomonas , and Weissella . The microbial composition of Tibetan kefir grains from different areas was found to be similar at the genus level in their study. The bacterial and fungal diversities in tarag, a special naturally fermented dairy product in Mongolia and the northwest of China, were investigated by Sun et al. ( 2014 2014 ) using pyrosequencing. A total of 47 bacterial and 43 fungal genera in 17 tarag samples were found, with Lactobacillus and Galactomyces being the predominant genera of bacteria and fungi, respectively. Their results showed that the microbial flora in different samples may be stratified by geographic region. Xu et al. ( 2015 2015 ) investigated the bacterial and fungal diversities in home-made yoghurts from two counties of Xinjiang Uygur Autonomous Region of China by pyrosequencing. Six bacterial and two fungal phyla, comprising 69 bacterial and 20 fungal genera, respectively, were found among the 22 samples tested. Firmicutes and Ascomycota were the dominant phyla, and as in the tarag samples, Lactobacillus was the predominant bacterial genus. However, the predominant fungal genus was Saccharomyces , not Galactomyces as in tarag. Their results indicated that the microbial communities of home-made yoghurts vary with their geographical origin and manufacturing process. Conclusions and future perspectives 1 Chinese traditional fermented foods are a significant part of the Chinese dietary lifestyle. However, most traditional foods are still produced empirically and spontaneously due to the lack of knowledge of the microbiota involved in fermentation, which makes it hard to manipulate the fermentation process. HTS has proven to be a powerful tool for exploring complex environmental microbiota and tracking fermentation processes. It has provided a comprehensive view of traditionally fermented foods, laying a foundation for manipulating the process of fermentation by using selected microbes and for improving the overall quality of fermented products while retaining their traditional traits. More traditional fermented foods will be subjected to HTS analysis in the future. However, due to the limitations of reference databases, most sequences may be annotated accurately only at the genus level and many will be aligned to uncultured microbes. Therefore, the reference databases need to be updated to obtain more accurate results, e.g. at the species level. The sequencing of whole genomes rather than 16S rRNA genes, i.e. metagenomics analysis, has also revolutionized the analysis of complex microbial diversities in environmental samples by providing comprehensive information on taxonomic relationships, functional genes, and metabolic pathways. This provides a very useful approach to investigate the relationship between microorganisms and food characteristics. In future, this technique is likely to become sufficiently cheap and accessible for the routine analysis of the microbial diversity of foods. References F Abram . Systems-based approaches to unravel multi-species microbial community functioning . Comput Struct Biotechnol J , 2015 . 13 24 - 32 . (Available from: http://dx.doi.org/10.1016/j.csbj.2014.11.009 http://dx.doi.org/10.1016/j.csbj.2014.11.009 ) 25750697 . Systems-based approaches to unravel multi-species microbial community functioning K Alain , J Querellou . Cultivating the uncultured: limits, advances and future challenges . Extremophiles , 2009 . 13 ( 4 ): 583 - 594 . (Available from: http://dx.doi.org/10.1007/s00792-009-0261-3 http://dx.doi.org/10.1007/s00792-009-0261-3 ) 19548063 . Cultivating the uncultured: limits, advances and future challenges J Amann . Die direkte zählung der wasserbakterien mittels des ultramikroskops . Centralbl Bakteriol , 1911 . 29 381 - 384 . (in German) . Die direkte zählung der wasserbakterien mittels des ultramikroskops M Ayrapetyan , TC Williams , JD Oliver . Bridging the gap between viable but non-culturable and antibiotic persistent bacteria . Trends Microbiol , 2015 . 23 ( 1 ): 7 - 13 . (Available from: http://dx.doi.org/10.1016/j.tim.2014.09.004 http://dx.doi.org/10.1016/j.tim.2014.09.004 ) 25449050 . Bridging the gap between viable but non-culturable and antibiotic persistent bacteria R Azat , Y Liu , W Li , 等 . Probiotic properties of lactic acid bacteria isolated from traditionally fermented Xinjiang cheese . J Zhejiang Univ-Sci B (Biomed & Biotechnol) , 2016 . 17 ( 8 ): 597 - 609 . (Available from: http://dx.doi.org/10.1631/jzus.B1500250 http://dx.doi.org/10.1631/jzus.B1500250 ) 27487805 . Probiotic properties of lactic acid bacteria isolated from traditionally fermented Xinjiang cheese NA Bokulich , DA Mills . Next-generation approaches to the microbial ecology of food fermentations . BMB Rep , 2012 . 45 ( 7 ): 377 - 389 . 22831972 . Next-generation approaches to the microbial ecology of food fermentations ZH Cao , DH Gu , QY Lin , 等 . Effect of pu-erh tea on body fat and lipid profiles in rats with diet-induced obesity . Phytother Res , 2011 . 25 ( 2 ): 234 - 238 . (Available from: http://dx.doi.org/10.1002/ptr.3247 http://dx.doi.org/10.1002/ptr.3247 ) 20641056 . Effect of pu-erh tea on body fat and lipid profiles in rats with diet-induced obesity Centers for Disease Control and Prevention Botulism outbreak associated with eating fermented food–Alaska, 2001 . MMWR Morb Mortal Wkly Rep , 2001 . 50 ( 32 ): 680 - 682 . 11785568 . Botulism outbreak associated with eating fermented food–Alaska, 2001 SH Chao , HY Huang , CH Chang , 等 . Microbial diversity analysis of fermented mung beans (Lu-Doh-Huang) by using pyrosequencing and culture methods . PLoS ONE , 2013 . 8 ( 5 ): e63816 (Available from: http://doi.org/10.1371/journal.pone.0063816 http://doi.org/10.1371/journal.pone.0063816 ) 23700436 . Microbial diversity analysis of fermented mung beans (Lu-Doh-Huang) by using pyrosequencing and culture methods L de Vuyst , V Schrijvers , S Paramithiotis , 等 . The biodiversity of lactic acid bacteria in greek traditional wheat sourdoughs is reflected in both composition and metabolite formation . Appl Environ Microbiol , 2002 . 68 ( 12 ): 6059 - 6069 . (Available from: http://dx.doi.org/10.1128/aem.68.12.6059-6069.2002 http://dx.doi.org/10.1128/aem.68.12.6059-6069.2002 ) 12450829 . The biodiversity of lactic acid bacteria in greek traditional wheat sourdoughs is reflected in both composition and metabolite formation L de Vuyst , S van Kerrebroeck , H Harth , 等 . Microbial ecology of sourdough fermentations: diverse or uniform? . Food Microbiol , 2014 . 37 11 - 29 . (Available from: http://dx.doi.org/10.1016/j.fm.2013.06.002 http://dx.doi.org/10.1016/j.fm.2013.06.002 ) 24230469 . Microbial ecology of sourdough fermentations: diverse or uniform? M Du , J Chen , X Zhang , 等 . Retention of virulence in a viable but nonculturable Edwardsiella tarda isolate . Appl Environ Microbiol , 2007 . 73 ( 4 ): 1349 - 1354 . (Available from: http://dx.doi.org/10.1128/AEM.02243-06 http://dx.doi.org/10.1128/AEM.02243-06 ) 17189433 . Retention of virulence in a viable but nonculturable Edwardsiella tarda isolate P Elizaquivel , A Pérez-Cataluña , A Yépez , 等 . Pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha , a traditional maize-based fermented beverage from Northwestern Argentina . Int J Food Microbiol , 2015 . 198 9 - 18 . (Available from: http://dx.doi.org/10.1016/j.ijfoodmicro.2014.12.027 http://dx.doi.org/10.1016/j.ijfoodmicro.2014.12.027 ) 25584777 . Pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha, a traditional maize-based fermented beverage from Northwestern Argentina D Ercolini , F de Filippis , A la Storia , 等 . “Remake” by high-throughput sequencing of the microbiota involved in the production of water buffalo mozzarella cheese . Appl Environ Microbiol , 2012 . 78 ( 22 ): 8142 - 8145 . (Available from: http://dx.doi.org/10.1128/AEM.02218-12 http://dx.doi.org/10.1128/AEM.02218-12 ) 22941080 . “Remake” by high-throughput sequencing of the microbiota involved in the production of water buffalo mozzarella cheese D Ercolini , E Pontonio , F de Filippis , 等 . Microbial ecology dynamics during rye and wheat sourdough preparation . Appl Environ Microbiol , 2013 . 79 ( 24 ): 7827 - 7836 . (Available from: http://dx.doi.org/10.1128/AEM.02955-13 http://dx.doi.org/10.1128/AEM.02955-13 ) 24096427 . Microbial ecology dynamics during rye and wheat sourdough preparation A Escobar-Zepeda , A Sanchez-Flores , MQ Baruch . Metagenomic analysis of a Mexican ripened cheese reveals a unique complex microbiota . Food Microbiol , 2016 . 57 116 - 127 . (Available from: http://dx.doi.org/10.1016/j.fm.2016.02.004 http://dx.doi.org/10.1016/j.fm.2016.02.004 ) 27052710 . Metagenomic analysis of a Mexican ripened cheese reveals a unique complex microbiota M Fakruddin , KSB Mannan , S Andrews . Viable but nonculturable bacteria: food safety and public health perspective . ISRN Microbiol , 2013 . 2013 ( 8113 ): 703813 (Available from: http://dx.doi.org/10.1155/2013/703813 http://dx.doi.org/10.1155/2013/703813 ) 24191231 . Viable but nonculturable bacteria: food safety and public health perspective RS Fang , YC Dong , F Chen , 等 . Bacterial diversity analysis during the fermentation processing of traditional Chinese yellow rice wine revealed by 16S rDNA 454 pyrosequencing . J Food Sci , 2015 . 80 ( 10 ): M2265 - M2271 . (Available from: http://dx.doi.org/10.1111/1750-3841.13018 http://dx.doi.org/10.1111/1750-3841.13018 ) 26409170 . Bacterial diversity analysis during the fermentation processing of traditional Chinese yellow rice wine revealed by 16S rDNA 454 pyrosequencing J Gao , F Gu , J He , 等 . Metagenome analysis of bacterial diversity in Tibetan kefir grains . Eur Food Res Technol , 2013 . 236 ( 3 ): 549 - 556 . (Available from: http://dx.doi.org/10.1007/s00217-013-1912-2 http://dx.doi.org/10.1007/s00217-013-1912-2 ) . Metagenome analysis of bacterial diversity in Tibetan kefir grains M Gobbetti , A Corsetti . Lactobacillus sanfrancisco a key sourdough lactic acid bacterium: a review . Food Microbiol , 1997 . 14 ( 2 ): 175 - 187 . (Available from: http://dx.doi.org/10.1006/fmic.1996.0083 http://dx.doi.org/10.1006/fmic.1996.0083 ) . Lactobacillus sanfrancisco a key sourdough lactic acid bacterium: a review ZB Guan , ZH Zhang , Y Cao , 等 . Analysis and comparison of bacterial communities in two types of ‘wheat Qu ’, the starter culture of Shaoxing rice wine, using nested PCR-DGGE . J Inst Brew , 2012 . 118 ( 1 ): 127 - 132 . (Available from: http://dx.doi.org/10.1002/jib.4 http://dx.doi.org/10.1002/jib.4 ) . Analysis and comparison of bacterial communities in two types of ‘wheat Qu’, the starter culture of Shaoxing rice wine, using nested PCR-DGGE WP Hammes , MG Gänzle . B.J.B Wood . Sourdough breads and related products . Microbiology of Fermented Foods , 1997 . Springer US , 199 - 216 . (Available from: http://dx.doi.org/10.1007/978-1-4613-0309-1_8 http://dx.doi.org/10.1007/978-1-4613-0309-1_8 ) . Sourdough breads and related products K Hayashi . PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA . PCR Methods Appl , 1991 . ( 1 ): 34 - 38 . 1842918 . PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA Y Hou , W Shao , R Xiao , 等 . Pu-erh tea aqueous extracts lower atherosclerotic risk factors in a rat hyperlipidemia model . Exp Gerontol , 2009 . 44 ( 6-7 ): 434 - 439 . (Available from: http://dx.doi.org/10.1016/j.exger.2009.03.007 http://dx.doi.org/10.1016/j.exger.2009.03.007 ) 19348878 . Pu-erh tea aqueous extracts lower atherosclerotic risk factors in a rat hyperlipidemia model H Hu , Y Xu , HP Lu , 等 . Evaluation of yeasts from Tibetan fermented products as agents for biocontrol of blue mold of Nashi pear fruits . J Zhejiang Univ-Sci B (Biomed & Biotechnol) , 2015 . 16 ( 4 ): 275 - 285 . (Available from: http://dx.doi.org/10.1631/jzus.B1400162 http://dx.doi.org/10.1631/jzus.B1400162 ) 25845361 . Evaluation of yeasts from Tibetan fermented products as agents for biocontrol of blue mold of Nashi pear fruits P Hugenholtz . Exploring prokaryotic diversity in the genomic era . Genome Biol , 2002 . 3 reviews0003 - 1 . (Available from: http://dx.doi.org/10.1186/gb-2002-3-2-reviews0003 http://dx.doi.org/10.1186/gb-2002-3-2-reviews0003 ) 11864374 . Exploring prokaryotic diversity in the genomic era S Jiang , J Lu , H Li . Comparative metatranscriptomic analysis of microbial communities at two different stages during the pile-fermentation of Puer tea . J Beijing Univ Chem Technol (Nat Sci Ed) , 2012 . 39 ( 6 ): 84 - 89 . (in Chinese) . Comparative metatranscriptomic analysis of microbial communities at two different stages during the pile-fermentation of Puer tea JY Jung , SH Lee , JM Kim , 等 . Metagenomic analysis of kimchi, a traditional Korean fermented food . Appl Environ Microbiol , 2011 . 77 ( 7 ): 2264 - 2274 . (Available from: http://dx.doi.org/10.1128/AEM.02157-10 http://dx.doi.org/10.1128/AEM.02157-10 ) 21317261 . Metagenomic analysis of kimchi, a traditional Korean fermented food JY Jung , SH Lee , HM Jin , 等 . Metatranscriptomic analysis of lactic acid bacterial gene expression during kimchi fermentation . Int J Food Microbiol , 2013 . 163 ( 2-3 ): 171 - 179 . (Available from: http://dx.doi.org/10.1016/j.ijfoodmicro.2013.02.022 http://dx.doi.org/10.1016/j.ijfoodmicro.2013.02.022 ) 23558201 . Metatranscriptomic analysis of lactic acid bacterial gene expression during kimchi fermentation T Kaeberlein , K Lewis , SS Epstein . Isolating “uncultivable” microorganisms in pure culture in a simulated natural environment . Science , 2002 . 296 ( 5570 ): 1127 - 1129 . (Available from: http://dx.doi.org/10.1126/science.1070633 http://dx.doi.org/10.1126/science.1070633 ) 12004133 . Isolating “uncultivable” microorganisms in pure culture in a simulated natural environment G Kergourlay , B Taminiau , G Daube , 等 . Metagenomic insights into the dynamics of microbial communities in food . Int J Food Microbiol , 2015 . 213 ( 20 ): 31 - 39 . (Available from: http://dx.doi.org/10.1016/j.ijfoodmicro.2015.09.010 http://dx.doi.org/10.1016/j.ijfoodmicro.2015.09.010 ) 26414193 . Metagenomic insights into the dynamics of microbial communities in food L Kline , T Sugihara . Microorganisms of the San Francisco sour dough bread process II. Isolation and characterization of undescribed bacterial species responsible for the souring activity . Appl Environ Microbiol , 1971 . 21 ( 3 ): 459 - 465 . . Microorganisms of the San Francisco sour dough bread process II. Isolation and characterization of undescribed bacterial species responsible for the souring activity A Ktenioudaki , L Alvarez-Jubete , TJ Smyth , 等 . Application of bioprocessing techniques (sourdough fermentation and technological aids) for brewer’s spent grain breads . Food Res Int , 2015 . 73 107 - 116 . (Available from: http://dx.doi.org/10.1016/j.foodres.2015.03.008 http://dx.doi.org/10.1016/j.foodres.2015.03.008 ) . Application of bioprocessing techniques (sourdough fermentation and technological aids) for brewer’s spent grain breads K Lewis , S Epstein , A D'Onofrio , 等 . Uncultured microorganisms as a source of secondary metabolites . J Antibiotics , 2010 . 63 ( 8 ): 468 - 476 . (Available from: http://dx.doi.org/10.1038/ja.2010.87 http://dx.doi.org/10.1038/ja.2010.87 ) . Uncultured microorganisms as a source of secondary metabolites P Li , H Liang , WT Lin , 等 . Microbiota dynamics associated with environmental conditions and potential roles of cellulolytic communities in traditional Chinese cereal starter solid-state fermentation . Appl Environ Microbiol , 2015 . 81 ( 15 ): 5144 - 5156 . (Available from: http://dx.doi.org/10.1128/aem.01325-15 http://dx.doi.org/10.1128/aem.01325-15 ) 26002897 . Microbiota dynamics associated with environmental conditions and potential roles of cellulolytic communities in traditional Chinese cereal starter solid-state fermentation XR Li , EB Ma , LZ Yan , 等 . Bacterial and fungal diversity in the traditional Chinese liquor fermentation process . Int J Food Microbiol , 2011 . 146 ( 1 ): 31 - 37 . (Available from: http://dx.doi.org/10.1016/j.ijfoodmicro.2011.01.030 http://dx.doi.org/10.1016/j.ijfoodmicro.2011.01.030 ) 21377228 . Bacterial and fungal diversity in the traditional Chinese liquor fermentation process XR Li , EB Ma , LZ Yan , 等 . Bacterial and fungal diversity in the starter production process of Fen liquor, a traditional Chinese liquor . J Microbiol , 2013 . 51 ( 4 ): 430 - 438 . (Available from: http://dx.doi.org/10.1007/s12275-013-2640-9 http://dx.doi.org/10.1007/s12275-013-2640-9 ) 23990293 . Bacterial and fungal diversity in the starter production process of Fen liquor, a traditional Chinese liquor Z Li , H Li , C Deng , 等 . Effect of Lactobacillus plantarum  DM616 on dough fermentation and Chinese steamed bread quality . J Food Process Pres , 2014 . 39 ( 1 ): 30 - 37 . (Available from: http://dx.doi.org/10.1111/jfpp.12205 http://dx.doi.org/10.1111/jfpp.12205 ) . Effect of Lactobacillus plantarum  DM616 on dough fermentation and Chinese steamed bread quality Z Ling , J Kong , P Jia , 等 . Analysis of oral microbiota in children with dental caries by PCR-DGGE and barcoded pyrosequencing . Microb Ecol , 2010 . 60 ( 3 ): 677 - 690 . (Available from: http://dx.doi.org/10.1007/s00248-010-9712-8 http://dx.doi.org/10.1007/s00248-010-9712-8 ) 20614117 . Analysis of oral microbiota in children with dental caries by PCR-DGGE and barcoded pyrosequencing SN Liu , Y Han , ZJ Zhou . Lactic acid bacteria in traditional fermented Chinese foods . Food Res Int , 2011 . 44 ( 3 ): 643 - 651 . (Available from: http://dx.doi.org/10.1016/j.foodres.2010.12.034 http://dx.doi.org/10.1016/j.foodres.2010.12.034 ) . Lactic acid bacteria in traditional fermented Chinese foods SP Liu , J Mao , YY Liu , 等 . Bacterial succession and the dynamics of volatile compounds during the fermentation of Chinese rice wine from Shaoxing region . World J Microbiol Biotechnol , 2015 . 31 ( 12 ): 1907 - 1921 . (Available from: http://dx.doi.org/10.1007/s11274-015-1931-1 http://dx.doi.org/10.1007/s11274-015-1931-1 ) 26492888 . Bacterial succession and the dynamics of volatile compounds during the fermentation of Chinese rice wine from Shaoxing region T Liu , Y Li , J Chen , 等 . Prevalence and diversity of lactic acid bacteria in Chinese traditional sourdough revealed by culture dependent and pyrosequencing approaches . LWT-Food Sci Technol , 2016 . 68 91 - 97 . (Available from: http://dx.doi.org/10.1016/j.lwt.2015.12.025 http://dx.doi.org/10.1016/j.lwt.2015.12.025 ) . Prevalence and diversity of lactic acid bacteria in Chinese traditional sourdough revealed by culture dependent and pyrosequencing approaches W Liu , Y Zheng , LY Kwok , 等 . High-throughput sequencing for the detection of the bacterial and fungal diversity in Mongolian naturally fermented cow’s milk in Russia . BMC Microbiol , 2015 . 15 45 (Available from: http://dx.doi.org/10.1186/s12866-015-0385-9 http://dx.doi.org/10.1186/s12866-015-0385-9 ) 25887414 . High-throughput sequencing for the detection of the bacterial and fungal diversity in Mongolian naturally fermented cow’s milk in Russia C Lyu , C Chen , F Ge , 等 . A preliminary metagenomic study of puer tea during pile fermentation . J Sci Food Agric , 2013 . 93 ( 13 ): 3165 - 3174 . (Available from: http://dx.doi.org/10.1002/jsfa.6149 http://dx.doi.org/10.1002/jsfa.6149 ) 23553377 . A preliminary metagenomic study of puer tea during pile fermentation ER Mardis . Next-generation DNA sequencing methods . Annu Rev Genomics Hum Genet , 2008 . 9 387 - 402 . (Available from: http://dx.doi.org/10.1146/annurev.genom.9.081307.164359 http://dx.doi.org/10.1146/annurev.genom.9.081307.164359 ) 18576944 . Next-generation DNA sequencing methods AJ Marsh , O O'Sullivan , C Hill , 等 . Sequencing-based analysis of the bacterial and fungal composition of kefir grains and milks from multiple sources . PLoS ONE , 2013 . 8 ( 7 ): e69371 (Available from: http://dx.doi.org/10.1371/journal.pone.0069371 http://dx.doi.org/10.1371/journal.pone.0069371 ) 23894461 . Sequencing-based analysis of the bacterial and fungal composition of kefir grains and milks from multiple sources TL Marsh . Terminal restriction fragment length polymorphism (T-RFLP): an emerging method for characterizing diversity among homologous populations of amplification products . Curr Opin Microbiol , 1999 . 2 ( 3 ): 323 - 327 . (Available from: http://dx.doi.org/10.1016/S1369-5274(99)80056-3 http://dx.doi.org/10.1016/S1369-5274(99)80056-3 ) 10383864 . Terminal restriction fragment length polymorphism (T-RFLP): an emerging method for characterizing diversity among homologous populations of amplification products B Mayo , CT Rachid , Á Alegría , 等 . Impact of next generation sequencing techniques in food microbiology . Curr Genomics , 2014 . 15 ( 4 ): 293 - 309 . (Available from: http://dx.doi.org/10.2174/1389202915666140616233211 http://dx.doi.org/10.2174/1389202915666140616233211 ) 25132799 . Impact of next generation sequencing techniques in food microbiology G Muyzer . DGGE/TGGE a method for identifying genes from natural ecosystems . Curr Opin Microbiol , 1999 . 2 ( 3 ): 317 - 322 . (Available from: http://dx.doi.org/10.1016/S1369-5274(99)80055-1 http://dx.doi.org/10.1016/S1369-5274(99)80055-1 ) 10383868 . DGGE/TGGE a method for identifying genes from natural ecosystems U Nalbantoglu , A Cakar , H Dogan , 等 . Metagenomic analysis of the microbial community in kefir grains . Food Microbiol , 2014 . 41 42 - 51 . (Available from: http://dx.doi.org/10.1016/j.fm.2014.01.014 http://dx.doi.org/10.1016/j.fm.2014.01.014 ) 24750812 . Metagenomic analysis of the microbial community in kefir grains Z Nie , Y Zheng , M Wang , 等 . Exploring microbial succession and diversity during solid-state fermentation of Tianjin duliu mature vinegar . Bioresour Technol , 2013 . 148 325 - 333 . (Available from: http://dx.doi.org/10.1016/j.biortech.2013.08.152 http://dx.doi.org/10.1016/j.biortech.2013.08.152 ) 24055975 . Exploring microbial succession and diversity during solid-state fermentation of Tianjin duliu mature vinegar JD Oliver . The viable but nonculturable state in bacteria . J Microbiol , 2005 . 43 ( 1 ): 93 - 100 . 15765062 . The viable but nonculturable state in bacteria EJ Park , KH Kim , GC Abell , 等 . Metagenomic analysis of the viral communities in fermented foods . Appl Environ Microb , 2011 . 77 ( 4 ): 1284 - 1291 . (Available from: http://dx.doi.org/10.1128/AEM.01859-10 http://dx.doi.org/10.1128/AEM.01859-10 ) . Metagenomic analysis of the viral communities in fermented foods Q Peng , Y Yang , Y Guo , 等 . Analysis of bacterial diversity during acetic acid fermentation of Tianjin duliu aged vinegar by 454 pyrosequencing . Curr Microbiol , 2015 . 71 ( 2 ): 195 - 203 . (Available from: http://dx.doi.org/10.1007/s00284-015-0823-9 http://dx.doi.org/10.1007/s00284-015-0823-9 ) 25903266 . Analysis of bacterial diversity during acetic acid fermentation of Tianjin duliu aged vinegar by 454 pyrosequencing H Piao , E Hawley , S Kopf , 等 . Insights into the bacterial community and its temporal succession during the fermentation of wine grapes . Front Microbiol , 2015 . 6 809 (Available from: http://dx.doi.org/10.3389/fmicb.2015.00809 http://dx.doi.org/10.3389/fmicb.2015.00809 ) 26347718 . Insights into the bacterial community and its temporal succession during the fermentation of wine grapes O Prakash , Y Shouche , K Jangid , 等 . Microbial cultivation and the role of microbial resource centers in the omics era . Appl Microbiol Biotechnol , 2013 . 97 ( 1 ): 51 - 62 . (Available from: http://dx.doi.org/10.1007/s00253-012-4533-y http://dx.doi.org/10.1007/s00253-012-4533-y ) 23138712 . Microbial cultivation and the role of microbial resource centers in the omics era L Quigley , O O'Sullivan , TP Beresford , 等 . High-throughput sequencing for detection of subpopulations of bacteria not previously associated with artisanal cheeses . Appl Environ Microbiol , 2012 . 78 ( 16 ): 5717 - 5723 . (Available from: http://dx.doi.org/10.1128/AEM.00918-12 http://dx.doi.org/10.1128/AEM.00918-12 ) 22685131 . High-throughput sequencing for detection of subpopulations of bacteria not previously associated with artisanal cheeses CS Riesenfeld , PD Schloss , J Handelsman . Metagenomics: genomic analysis of microbial communities . Annu Rev Genet , 2004 . 38 525 - 552 . (Available from: http://dx.doi.org/10.1146/annurev.genet.38.072902.091216 http://dx.doi.org/10.1146/annurev.genet.38.072902.091216 ) 15568985 . Metagenomics: genomic analysis of microbial communities N Shaffer , RB Wainwright , JP Middaugh , 等 . Botulism among alaska natives. The role of changing food preparation and consumption practices . West J Med , 1990 . 153 ( 4 ): 390 - 393 . 2244373 . Botulism among alaska natives. The role of changing food preparation and consumption practices G Shi . Talk about Chinese vinegar . China Brew , 1999 . 6 39 - 40 . (in Chinese) . Talk about Chinese vinegar M Simonen , I Palva . Protein secretion in Bacillus species . Microbiol Mol Biol Rev , 1993 . 57 ( 1 ): 109 - 137 . . Protein secretion in Bacillus species G Smit , BA Smit , WJ Engels . Flavour formation by lactic acid bacteria and biochemical flavour profiling of cheese products . FEMS Microbiol Rev , 2005 . 29 ( 3 ): 591 - 610 . (Available from: http://dx.doi.org/10.1016/j.femsre.2005.04.002 http://dx.doi.org/10.1016/j.femsre.2005.04.002 ) 15935512 . Flavour formation by lactic acid bacteria and biochemical flavour profiling of cheese products EJ Stewart . Growing unculturable bacteria . J Bacteriol , 2012 . 194 ( 16 ): 4151 - 4160 . (Available from: http://dx.doi.org/10.1128/JB.00345-12 http://dx.doi.org/10.1128/JB.00345-12 ) 22661685 . Growing unculturable bacteria Z Sun , W Liu , Q Bao , 等 . Investigation of bacterial and fungal diversity in tarag using high-throughput sequencing . J Dairy Sci , 2014 . 97 ( 10 ): 6085 - 6096 . (Available from: http://dx.doi.org/10.3168/jds.2014-8360 http://dx.doi.org/10.3168/jds.2014-8360 ) 25129502 . Investigation of bacterial and fungal diversity in tarag using high-throughput sequencing W Tesfaye , ML Morales , M García-Parrilla , 等 . Wine vinegar: technology, authenticity and quality evaluation . Trends Food Sci Technol , 2002 . 13 ( 1 ): 12 - 21 . (Available from: http://dx.doi.org/10.1016/S0924-2244(02)00023-7 http://dx.doi.org/10.1016/S0924-2244(02)00023-7 ) . Wine vinegar: technology, authenticity and quality evaluation J Tian , Z Zhu , B Wu , 等 . Bacterial and fungal communities in Pu’er tea samples of different ages . J Food Sci , 2013 . 78 ( 8 ): M1249 - M1256 . (Available from: http://dx.doi.org/10.1111/1750-3841.12218 http://dx.doi.org/10.1111/1750-3841.12218 ) 23957415 . Bacterial and fungal communities in Pu’er tea samples of different ages JC Venter , K Remington , JF Heidelberg , 等 . Environmental genome shotgun sequencing of the Sargasso sea . Science , 2004 . 304 ( 5667 ): 66 - 74 . (Available from: http://dx.doi.org/10.1126/science.1093857 http://dx.doi.org/10.1126/science.1093857 ) 15001713 . Environmental genome shotgun sequencing of the Sargasso sea CD Wang , Q Chen , Q Wang , 等 . Long-term batch brewing accumulates adaptive microbes, which comprehensively produce more flavorful Chinese liquors . Food Res Int , 2014 . 62 894 - 901 . (Available from: http://dx.doi.org/10.1016/j.foodres.2014.05.017 http://dx.doi.org/10.1016/j.foodres.2014.05.017 ) . Long-term batch brewing accumulates adaptive microbes, which comprehensively produce more flavorful Chinese liquors P Wang , J Mao , X Meng , 等 . Changes in flavour characteristics and bacterial diversity during the traditional fermentation of Chinese rice wines from Shaoxing region . Food Control , 2014 . 44 58 - 63 . (Available from: http://dx.doi.org/10.1016/j.foodcont.2014.03.018 http://dx.doi.org/10.1016/j.foodcont.2014.03.018 ) . Changes in flavour characteristics and bacterial diversity during the traditional fermentation of Chinese rice wines from Shaoxing region ZM Wang , ZM Lu , YJ Yu , 等 . Batch-to-batch uniformity of bacterial community succession and flavor formation in the fermentation of Zhenjiang aromatic vinegar . Food Microbiol , 2015 . 50 64 - 69 . (Available from: http://dx.doi.org/10.1016/j.fm.2015.03.012 http://dx.doi.org/10.1016/j.fm.2015.03.012 ) 25998816 . Batch-to-batch uniformity of bacterial community succession and flavor formation in the fermentation of Zhenjiang aromatic vinegar S Weckx , R van der Meulen , J Allemeersch , 等 . Community dynamics of bacteria in sourdough fermentations as revealed by their metatranscriptome . Appl Environ Microbiol , 2010 . 76 ( 16 ): 5402 - 5408 . (Available from: http://dx.doi.org/10.1128/AEM.00570-10 http://dx.doi.org/10.1128/AEM.00570-10 ) 20581179 . Community dynamics of bacteria in sourdough fermentations as revealed by their metatranscriptome S Weckx , J Allemeersch , R van der Meulen , 等 . Metatranscriptome analysis for insight into whole-ecosystem gene expression during spontaneous wheat and spelt sourdough fermentations . Appl Environ Microbiol , 2011 . 77 ( 2 ): 618 - 626 . (Available from: http://dx.doi.org/10.1128/AEM.02028-10 http://dx.doi.org/10.1128/AEM.02028-10 ) 21097589 . Metatranscriptome analysis for insight into whole-ecosystem gene expression during spontaneous wheat and spelt sourdough fermentations CL Wei , SH Chao , WB Tsai , 等 . Analysis of bacterial diversity during the fermentation of inyu , a high-temperature fermented soy sauce, using nested PCR-denaturing gradient gel electrophoresis and the plate count method . Food Microbiol , 2013 . 33 ( 2 ): 252 - 261 . (Available from: http://dx.doi.org/10.1016/j.fm.2012.10.001 http://dx.doi.org/10.1016/j.fm.2012.10.001 ) 23200659 . Analysis of bacterial diversity during the fermentation of inyu, a high-temperature fermented soy sauce, using nested PCR-denaturing gradient gel electrophoresis and the plate count method H Winterberg . Zur methodik der bakterienzählung . Zeitschr f Hygiene , 1898 . 29(1):75-93 ( in German ): 75 - 93 (in German) . (Available from: http://dx.doi.org/10.1007/BF02217377 http://dx.doi.org/10.1007/BF02217377 ) . Zur methodik der bakterienzählung G Xie , L Wang , Q Gao , 等 . Microbial community structure in fermentation process of Shaoxing rice wine by illumina-based metagenomic sequencing . J Sci Food Agric , 2013 . 93 ( 12 ): 3121 - 3125 . (Available from: http://dx.doi.org/10.1002/jsfa.6058 http://dx.doi.org/10.1002/jsfa.6058 ) 23553745 . Microbial community structure in fermentation process of Shaoxing rice wine by illumina-based metagenomic sequencing H Xu , W Liu , Q Gesudu , 等 . Assessment of the bacterial and fungal diversity in home-made yoghurts of Xinjiang, China by pyrosequencing . J Sci Food Agric , 2015 . 95 ( 10 ): 2007 - 2015 . (Available from: http://dx.doi.org/10.1002/jsfa.6912 http://dx.doi.org/10.1002/jsfa.6912 ) 25214028 . Assessment of the bacterial and fungal diversity in home-made yoghurts of Xinjiang, China by pyrosequencing W Xu , Z Huang , X Zhang , 等 . Monitoring the microbial community during solid-state acetic acid fermentation of Zhenjiang aromatic vinegar . Food Microbiol , 2011 . 28 ( 6 ): 1175 - 1181 . (Available from: http://dx.doi.org/10.1016/j.fm.2011.03.011 http://dx.doi.org/10.1016/j.fm.2011.03.011 ) 21645817 . Monitoring the microbial community during solid-state acetic acid fermentation of Zhenjiang aromatic vinegar XP Yang , JF Luo , L Xin , 等 . Microbial community structure and change during solid fermentation of Pu-erh tea . Food Sci , 2013 . 34 ( 19 ): 142 - 147 . (in Chinese) . Microbial community structure and change during solid fermentation of Pu-erh tea G Zhang , G He . Predominant bacteria diversity in Chinese traditional sourdough . J Food Sci , 2013 . 78 ( 8 ): M1218 - M1223 . (Available from: http://dx.doi.org/10.1111/1750-3841.12193 http://dx.doi.org/10.1111/1750-3841.12193 ) 23957410 . Predominant bacteria diversity in Chinese traditional sourdough G Zhang , FA Sadiq , L Zhu , 等 . Investigation of microbial communities of Chinese sourdoughs using culture-dependent and DGGE approaches . J Food Sci , 2015 . 80 ( 11 ): M2535 - M2542 . (Available from: http://dx.doi.org/10.1111/1750-3841.13093 http://dx.doi.org/10.1111/1750-3841.13093 ) 26461081 . Investigation of microbial communities of Chinese sourdoughs using culture-dependent and DGGE approaches GH Zhang , T Wu , FA Sadiq , 等 . A study revealing the key aroma compounds of steamed bread made by Chinese traditional sourdough . J Zhejiang Univ-Sci B (Biomed & Biotechnol) , 2016 . 17 ( 10 ): 787 - 797 . (Available from: http://dx.doi.org/10.1631/jzus.B1600130 http://dx.doi.org/10.1631/jzus.B1600130 ) 27704748 . A study revealing the key aroma compounds of steamed bread made by Chinese traditional sourdough JC Zhang , WJ Liu , ZH Sun , 等 . Diversity of lactic acid bacteria and yeasts in traditional sourdoughs collected from western region in Inner Mongolia of China . Food Control , 2011 . 22 ( 5 ): 767 - 774 . (Available from: http://dx.doi.org/10.1016/j.foodcont.2010.11.012 http://dx.doi.org/10.1016/j.foodcont.2010.11.012 ) . Diversity of lactic acid bacteria and yeasts in traditional sourdoughs collected from western region in Inner Mongolia of China X Zhang , J Zhao , X Du . Barcoded pyrosequencing analysis of the bacterial community of Daqu for light-flavour Chinese liquor . Lett Appl Microbiol , 2014 . 58 ( 6 ): 549 - 555 . (Available from: http://dx.doi.org/10.1111/lam.12225 http://dx.doi.org/10.1111/lam.12225 ) 24471485 . Barcoded pyrosequencing analysis of the bacterial community of Daqu for light-flavour Chinese liquor Z Zhang , Z Guan , X Liang , 等 . Establishment of PCR-DGGE for analysing the bacterial community of Shaoxing rice wine wheat Qu . Sci Technol Food Ind , 2012 . 33 ( 14 ): 206 - 209 . 206 (in Chinese) . Establishment of PCR-DGGE for analysing the bacterial community of Shaoxing rice wine wheat Qu M Zhao , DL Zhang , XQ Su , 等 . An integrated metagenomics/metaproteomics investigation of the microbial communities and enzymes in solid-state fermentation of Pu-erh tea . Sci Rep , 2015 . 5 10117 (Available from: http://dx.doi.org/10.1038/srep10117 http://dx.doi.org/10.1038/srep10117 ) 25974221 . An integrated metagenomics/metaproteomics investigation of the microbial communities and enzymes in solid-state fermentation of Pu-erh tea Q Zheng , B Lin , Y Wang , 等 . Proteomic and high-throughput analysis of protein expression and microbial diversity of microbes from 30-and 300-year pit muds of Chinese Luzhou-flavor liquor . Food Res Int , 2015 . 75 305 - 314 . (Available from: http://dx.doi.org/10.1016/j.foodres.2015.06.029 http://dx.doi.org/10.1016/j.foodres.2015.06.029 ) . Proteomic and high-throughput analysis of protein expression and microbial diversity of microbes from 30-and 300-year pit muds of Chinese Luzhou-flavor liquor Compliance with ethics guidelines Guo-qing HE, Tong-jie LIU, Faizan A. SADIQ, Jing-si GU, and Guo-hua ZHANG declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects performed by any of the authors. 10.1631/jzus.B1600148 Project supported by the National Natural Science Foundation of China (Nos. 31371826 and 31571808) and the China Postdoctoral Science Foundation Funded Project (No. 2016M592002) 2016-04-03 2017-03-13 2016-06-27 2022-01-05 Copyright © Zhejiang University and Springer-Verlag Berlin Heidelberg 2017 2017 Journal of Zhejiang University-SCIENCE B (Biomedicine and Biotechnology) 04 18