Inverse associations of outdoor activity and vitamin D intake with the risk of Parkinson’s disease

Inverse associations of outdoor activity and vitamin D intake with the risk of Parkinson’s disease 户外活动和维生素D的摄入量与帕金森病的风险呈负相关 1 first-author 2 3 4 4 1 corresp ‡Corresponding Author ‡Corresponding Author †E-mail:dwangwz@163.com †E-mail:dwangwz@163.com dwangwz@163.com Department of Neurology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China Department of Neurology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China Genome Analysis Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China Genome Analysis Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China Department of Rehabilitation, the First Affiliated Hospital, Harbin Medical University, Harbin 150001, China Department of Rehabilitation, the First Affiliated Hospital, Harbin Medical University, Harbin 150001, China Department of Neurology, the First Affiliated Hospital, Harbin Medical University, Harbin 150001, China Department of Neurology, the First Affiliated Hospital, Harbin Medical University, Harbin 150001, China Early studies had suggested that vitamin D intake was inversely associated with neurodegenerative diseases, such as Alzheimer’s disease and multiple sclerosis. However, the associations of vitamin D intake and outdoor activities with Parkinson’s disease (PD) are still unclear, so this study is to evaluate these relationships from a case-control study in elderly Chinese. The study population involved 209 cases with new onsets of PD and 210 controls without neurodegenerative diseases. The data on dietary vitamin D and outdoor activities were collected using a food-frequency questionnaire and self-report questionnaire. Multivariable logistic regressions were used to examine the associations between dietary outdoor activities, vitamin D intake and PD. Adjustment was made for sex, age, smoking, alcohol use, education, and body mass index (BMI). Adjusted odds ratios (ORs) for PD in quartiles for outdoor physical activity were 1 (reference), 0.739 (0.413, 1.321), 0.501 (0.282, 0.891), and 0.437 (0.241, 0.795), respectively ( P =0.002 for trend). Adjusted ORs for PD in quartiles for total vitamin D intake were 1 (reference), 0.647 (0.357, 1.170), 0.571 (0.318, 1.022), and 0.538 (0.301, 0.960), respectively ( P =0.011 for trend). Our study suggested that outdoor activity and total vitamin D intake were inversely associated with PD, and outdoor activity seems to be more significantly associated with decreased risk for PD. 研究目的 2 评估户外活动、维生素D的摄入与帕金森病（PD）风险关系。 研究方法 2 收集209例新发PD病例和210名无神经退行性疾病对照人群，进行食物频率问卷调查，计算膳食维生素D摄入量并记录自我报告的问卷调查户外活动情况。利用多变量Logistic回归研究膳食维生素D摄入及户外活动与PD的相关性，并校正性别、年龄、吸烟、饮酒、教育程度和身体质量指数（BMI）变量。按户外活动时间从少到多四分位分组，校正后的比值比（OR）（95%可信区间（CI））值分别为1（参考）、0.739（0.413，1.321）、0.501（0.282，0.891）和0.437（0.241，0.795），趋势 P =0.002；按维生素D摄入量从少到多四分位分组，校正后的OR值分别为1（参考）、0.647（0.357，1.170）、0.571（0.318，1.022）和0.538（0.301，0.960），趋势 P =0.011。 重要结论 2 表明户外活动和总的维生素D摄入量与PD呈负相关；户外活动可更显著下降PD的风险相关。 Outdoor activity Vitamin D intake Parkinson’s disease 户外活动 维生素D摄入量 帕金森氏病 Introduction 1 Parkinson’s disease (PD) is a common progressive neurodegenerative disorder, causing disability in the elderly (Voelker, 2006 2006 ; Sehanovic et al., 2011 2011 ). Although the pathogenesis remains elusive, environmental factors for PD have been proposed to be connected with the origin of this disease (Lees et al., 2009 2009 ; Liu et al., 2011 2011 ). Specific nutrients and food intake habits have been investigated as potential risk factors or protectors for PD (Anderson et al., 1999 1999 ; Ross et al., 2000 2000 ; Park et al., 2005 2005 ; Gao et al., 2007 2007 ; Jiang et al., 2014 2014 ). Vitamin D plays an important role in the pathogenesis of chronic diseases (e.g., cancer, cardiovascular diseases, and diabetes) and some progressive neurodegenerative disorders (such as Alzheimer’s disease and multiple sclerosis) (Knekt et al., 2010 2010 ; Annweiler et al., 2013 2013 ). Recently, chronically inadequate vitamin D was proposed to play a significant role in the pathogenesis of PD (van den Bos et al., 2013 2013 ). According to the suggested biological mechanism, the PD may be caused by a continuously inadequate vitamin D status, leading to a chronic loss of dopaminergic neurons in the brain (Knekt et al., 2010 2010 ). However, this finding was not reported for the Chinese population. Outdoor activities were also reported to be linked to PD (Kenborg et al., 2011 2011 ). Additionally, the dynamic changes of serum vitamin D are determined by dietary and sun avoidance factors, as 20% vitamin D is obtained from food (eggs, fish, animal liver, and dairy products), while 80% is from skin synthesis as a product of skin 7-dehydrocholesterol transformations induced by ultraviolet radiation (American Diabetes Association, 2007 2007 ; Marcinowska-Suchowierska et al., 2010 2010 ). Therefore, besides the benefit of outdoor activity per se , outdoor activity could also influence vitamin D status. These two factors should be both considered in one study; however, the epidemiological study on the relationships between intake of vitamin D, outdoor activities and the risk of PD was still limited. In this study, we evaluated the relationships between intake of vitamin D, outdoor activities and the risk of PD using data from a relatively large-scale case-control study in a Chinese population. Materials and methods 1 Two hundred and nine PD cases with new onsets and 210 age and sex matched healthy controls were included in this study at a clinic in the First and Second Affiliated Hospitals of Harbin Medical University and three community hospitals between Nov. 1, 2012 and Mar. 1, 2013 in Harbin in northern China. PD was diagnosed by the neurologists according to the UK PD Society Brain Bank clinical diagnostic criteria. Inclusion criteria were a new onset of PD and no other neurodegenerative diseases. The study protocol was approved by the Ethics Committee of the Harbin Medical University. Each participant was informed about the study and signed a consent form. All participants completed a questionnaire-based interview and received a physical examination. Investigators were trained in administering the questionnaire and physical examination before the survey. Data of each patient on educational status, outdoor physical activity (hours per week), smoking and drinking status, and medical history were collected using a face-to-face questionnaire answered by the participants. Dietary intakes were measured with a validated semi-quantitative food-frequency questionnaire (FFQ) (Li et al., 2010 2010 ). Dietary vitamin D intakes were calculated using the Food Nutrition Calculator (V1.60; the Centers for Disease Control of China). A questionnaire on the use of vitamin D as a supplement was administered. The information on using vitamin D supplements included the time and dosage of the supplements. Total vitamin D was calculated as dietary plus supplemented vitamin D. Weight and height were measured to the nearest 0.1 kg and 0.1 cm, respectively. Blood pressure was measured with a standard mercury sphygmomanometer. Body mass index (BMI) was calculated as weight (kg) divided by the square of height (m). Data were presented as mean±standard deviation (SD) or percentages as appropriate. Outdoor physical activity and total dietary vitamin D intake were categorized at quartile points, respectively. Multivariable logistic regression models were developed to evaluate the associations between dietary vitamin D, outdoor activity and PD after adjustment for age, sex, education, BMI, alcohol use, and smoking. Estimations of odds ratios (ORs) and their 95% confidence intervals (CIs) of PD for each category of the total vitamin D intake in comparison with the lowest category were assessed. The significance level was set at P < 0.05 and tests were two-tailed for all statistical analyses in this study. Results and discussion 1 There were no significant differences between the case and control groups in age, sex, education, percentage of current smoking, alcohol use, or indoor activity (Table 1 1 ). Compared with control subjects, PD cases had a significant higher BMI and lower total vitamin D intakes and outdoor physical activity hours. For outdoor physical activity, all the subjects claimed to walk and perform slow aerobic exercise. Table 2 2 presents crude and adjusted ORs and 95% CIs for the risk of PD associated with outdoor physical activity, which was grouped in quartiles: 1, 0–2 h/week; 2, 2–4 h/week; 3, 4–6 h/week; and 4, > 6 h/week. In the crude analysis, the inverse associations between the outdoor physical activity in the third and fourth quartiles and PD were significant ( P =0.001 for trend). After adjustment for age, sex, alcohol use, smoking, education, BMI, and total vitamin D intake, the adjusted ORs for PD in quartiles for outdoor physical activity were 1 (reference), 0.739 (0.413, 1.321), 0.501 (0.282, 0.891), and 0.437 (0.241, 0.795), respectively ( P =0.002 for trend). For total vitamin D intake in this crude analysis, inverse association was observed between total vitamin D intake and the risk of PD. Total vitamin D intake was categorized in quartiles: 1, 0–5 μg/d; 2, 5–8 μg/d; 3, 8–12 μg/d; and 4, > 12 μg/d. A negative association between vitamin D intake and PD was observed only at the top quartile. When adjusted for age, sex, alcohol use, smoking, education, BMI, and outdoor physical activity, adjusted ORs for PD in the quartiles for total vitamin D intake were 1 (reference), 0.647 (0.357, 1.170), 0.571 (0.318, 1.022), and 0.538 (0.301, 0.960), respectively ( P =0.011 for trend). As outdoor physical activity and total vitamin D intake were included in the same model of multiple logistics, it seems that outdoor physical activity was more strongly associated with a lower risk for PD. To investigate whether there is a joint effect of vitamin D and physical activity on the risk for PD, we analyzed these variables in the same model, but did not find a significant effect of vitamin D×physical activity ( P =0.606; Table 3 3 ). This finding indicated that both vitamin D and physical activity might have independent effects on PD. Data are expressed as mean±SD or number of subjects (percentage). Differences between cases and controls were assessed using a t -test for continuous data and χ 2 test for binary data Parameter Case ( n =209) Control ( n =210) P Age (year) 64.6±9.4 66.0±8.1 0.10 Male/female 73/136 76/134 0.84 Current smoking 50 (23.9%) 54 (25.7%) 0.74 Alcohol use 50 (23.9%) 60 (28.6%) 0.32 Education  <9 years 87 (41.6%) 83 (39.5%) 0.35  9–12 years 74 (35.4%) 66 (31.4%)  >12 years 48 (23.0%) 61 (29.0%) BMI (kg/m 2 ) 24.8±2.5 23.9±2.1 <0.01 Outdoor activity (h/week) 3.9±3.1 5.0±3.5 0.001 Indoor activity (h/week) 13.2±1.2 12.9±1.9 0.10 Vitamin D intake (g/d) 8.4±4.3 9.6±4.7 0.005 Quartile 1 as reference. n case / n control : the number of cases to the number of controls. Adjusted OR: adjusted for sex, age, smoking, alcohol use, education, and BMI 2 Quartile 4 Outdoor activity 4 Total vitamin D intake Time (h/week) n case / n control Crude OR (95% CI) Adjusted OR (95% CI) Intake (μg/d) n case / n contro Crude OR (95% CI) Adjusted OR (95% CI) 1 <2 63/40 1 1 <5 62/44 1 1 2 2–4 56/49 0.748 (0.427, 1.311) 0.739 (0.413, 1.321) 5–8 51/52 0.589 (0.331, 1.049) 0.647 (0.357, 1.170) 3 4–6 47/57 0.550 (0.313, 0.967) 0.501 (0.282, 0.891) 8–12 52/63 0.568 (0.319, 1.009) 0.571 (0.318, 1.022) 4 >6 42/64 0.388 (0.218, 0.692) 0.437 (0.241, 0.795) >12 43/61 0.505 (0.288, 0.887) 0.538 (0.301, 0.960) 9 P for trend 0.001 0.002 0.021 0.011 n case / n control : the number of cases to the number of controls. Adjusted OR: adjusted for sex, age, smoking, alcohol use, education, and BMI 2 Level 3 Outdoor activity 3 Total vitamin D intake 2 Vitamin D intake×outdoor activity OR (95% CI) Time (h/week) n case / n control Adjusted OR (95% CI) Intake (μg/d) n case / n control Adjusted OR (95% CI) Reference <4 119/89 1 <8 62/44 1 Higher level >4 89/121 0.494 (0.279, 0.877) >8 51/52 0.613 (0.348, 1.080) 1.231 (0.559, 2.711) 8 P for trend 0.016 0.09 0.606 As for the outdoor activity, Kenborg et al. ( 2011 2011 ) recently reported that men working outdoors have a lower risk of PD, which is in line with our results. The mechanism of this finding was still unclear. However, the recent study has indicated that greater intensity of physical activity was associated with a shorter time to complete the trail-making test and higher levels of verbal fluency, and positively correlated with brain volume, white matter, as well as a parietal lobe gray matter volume situated bilaterally at the precuneus in the older human (Xu et al., 2010 2010 ; Speelman et al., 2011 2011 ; Abrantes et al., 2012 2012 ). Moreover, improved vitamin D status through sunlight during outdoor physical activity could be another potential factor for its protective role in development of PD. As serum vitamin D is related to dietary and sun avoidance (Marcinowska-Suchowierska et al., 2010 2010 ), outdoor activity might have multiple roles in the protection of PD. In line with our findings, lower serum vitamin D was also significantly associated with an increased risk of PD, whereas supplemental intake of vitamin D was related to a lower risk of PD (Knekt et al., 2010 2010 ; van den Bos et al., 2013 2013 ). However, no material associations between them were found in another case-control study (Miyake et al., 2011 2011 ). Considering the mild association between vitamin D intake and PD detected in our larger population, we think that a smaller sample size and unmatched controls might contribute to the insignificant association in previous studies. Larger cohort population studies with controlled confounders are needed to validate this issue. The mechanisms of how vitamin D protects against PD are not clear. One possible explanation is that vitamin D exhibits neuroprotective effects, which might be due to its anti-oxidative mechanisms, neuronal calcium regulation, immunomodulation, enhanced nerve conduction, and detoxification mechanisms (Buell and Dawson-Hughes, 2008 2008 ). The chronic inadequacy of vitamin D leads to the loss of dopaminergic neurons in the substantia nigra region and causes the further development of PD. Another explanation might be the insulin resistance. Vitamin D and physical activity are both inversely associated with poor insulin resistance, and insulin resistance has been accepted as a link with PD. Individuals with vitamin D deficiency are at an increasing risk for dyslipidemia, insulin resistance, and type 2 diabetes (Holick, 2007 2007 ). In addition, outdoor activity could also improve insulin resistance. Thus, outdoor activity and vitamin D intake might both protect PD through maintaining normal and stable insulin sensitivity. Before this study, we have discussed and considered the possible confounding variables in this study. We all agreed that some important factors, such as vitamin D supplements, outdoor activity, age, BMI, smoking, drinking, education, foods rich in vitamin D, living location, and traveling to other places, could influence the vitamin D status. Thus, we selected the three living locations (communities) with similar buildings and sun exposure time. Subjects always traveling to southern places were excluded. And the other factors were all considered (age and sex were matched between groups) and adjusted in the analysis. So we think we could gain a more confident finding regarding the confounding factors control. Additionally, this study included a relatively larger population, which would give us a more stable result and statistical power. However, there are some limitations to be considered. First, the small number of cases and case-control study design may still lead to instable results. Second, absent measurement of serum 25-hydroxyvitamin D (25-OHD) is another limitation in this study. Although poor dietary habits and outdoor physical activity could contribute to the vitamin D deficiency/insufficiency, but the lack of data on serum 25-OHD may not fully represent the vitamin D status in this study. Thus, readers should take the results with some caution. More accurate measurements should be used in cohort studies. In conclusion, our findings from this Chinese case-control study suggest that outdoor activity and vitamin D intake are both inversely associated with PD, and outdoor activity seems to have a stronger association. References AM Abrantes , JH Friedman , RA Brown , 等 . Physical activity and neuropsychiatric symptoms of Parkinson disease . 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Additional informed consent was obtained from all patients for which identifying information is included in this article. 10.1631/jzus.B1400005 2014-01-03 2014-07-25 2014-06-11 2022-01-05 Copyright © Zhejiang University and Springer-Verlag Berlin Heidelberg 2014 2014 Journal of Zhejiang University-SCIENCE B (Biomedicine and Biotechnology) 10 15