Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin

Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin first-author corresp ‡Corresponding Author ‡Corresponding Author †E-mail:shangzc@mail.hz.zj.cn †E-mail:shangzc@mail.hz.zj.cn shangzc@mail.hz.zj.cn Department of Chemistry, Zhejiang University, Hangzhou 310027, China Department of Chemistry, Zhejiang University, Hangzhou 310027, China The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy. Pefloxacin mesylate (PFLX) Bovine lactoferrin (BLf) Human serum albumin (HSA) Fluorescence spectra Energy-transfer efficiency INTRODUCTION 1 Pefloxacin mesylate (PFLX, Fig. 1 1 ) is an antibiotic in a class of drugs called fluoroquinolones with wide spectrum of activity against various bacterial infections, such as bronchitis and urinary tract infections (Blandeau, 1999 1999 ; Wang and Yuan, 1999 1999 ). Due to its advantages such as good tolerance, the intaking of PFLX has no adverse effect on the pharmacokinetics features, low photo toxicity and intensive and effective antibacterial activity compared to the common quinolones, it has been widely used in clinical practice. However, few reports on the binding of PFLX to bovine lactoferrin (BLf) and human serum albumin (HSA) are available in the literature, so a detailed study on the binding reaction of PFLX will be of great interest to scientists in general and clinicians in particular. Lactoferrin is an 80 kDa iron-binding glycoprotein that is present in several biological fluids and in the secretory granules of neutrophils (Masson et al., 1966 1966 ; 1969 1969 ; Aguila and Brock, 2001 2001 ) and is associated with a wide variety of biologically important processes, including host defense, regulation of cell growth, and cell differentiation (Sanchez et al., 1992 1992 ). Lactoferrin is folded into two approximately equal lobes (N- and C-terminal), each of which is split by the iron-binding cleft into two domains (N1, N2 and C1, C2 respectively) (Baker et al., 1998 1998 ). It is a highly basic protein, consequently interacts with many acidic molecules (Lampreave et al., 1990 1990 ) to possibly modify the biological properties of lactoferrin. Serum albumins are major soluble protein constituents of the circulatory system and have many physiological functions. The most outstanding function of albumins is that they serve as a depot protein and a transport protein for numerous endogenous and exogenous compounds. The exogenous substances bound with high affinity to protein are drugs. This interaction between protein and drug molecules results in formation of a stable protein-drug complex. Studying binding phenomena will be important for interpretation of the metabolism and transporting process, and will help to explain the relationship between structures and functions of protein. Fluorescence spectroscopy is an appropriate method to determine the interaction between the small molecule ligand and bio-macromolecule. From measurement and analysis of the emission peak, the transfer efficiency of energy, the lifetime, and fluorescence polarization, etc., a vast amount of information will be yielded on the structural fluctuations and the microenvironment surrounding the fluorophore in the macromolecule. In this work, the binding reactions between pefloxacin mesylate and bovine lactoferrin (BLf) and human serum albumin (HSA) were investigated and the binding parameters and transfer efficiency of energy were also measured. Another main goal of this work was to check the effect of pefloxacin mesylate on BLf and HSA conformational changes. Based on the site-binding model (Klotz and Hunstone, 1971 1971 ; Congdon et al., 1993 1993 ; Baptista and Indig, 1998 1998 ; Scatchard, 1949 1949 ), practical fitting formulas for drugs binding to BLf and HSA were proposed. The results showed that these models can match very well with the experiments data. EXPERIMENTAL DETAILS 1 Reagents and apparatus 2 Solutions of BLf (1.00×10 −5 mol/L), HSA (1.00×10 −5 mol/L) and pefloxacin mesylate (5.0×10 −4 mol/L) and 0.05 mol/L Tris-HCl buffer pH=7.4 (0.1 mol/L NaCl used to keep the ionic strength constant) were prepared. All reagents except trimethylolmethane(tris), which was biochemical reagent were of analytical grade and double-distilled water was used throughout. All of the fluorescence measurements were carried out on an F-4500 recording spectrofluorimeter (Hitachi, Japan) equipped with a xenon lamp source and 1.0 cm cells. A UV-260 recording spectrophotometer (Shimadzu, Japan) was used for scanning the UV spectrum. All pH measurements were made with a pHS-3C digital pH-meter (Yitong company of Jintan, Jiangsu, China). Procedures 2 BLf and pefloxacin mesylate along with HSA and pefloxacin mesylate were dissolved in Tris-HCl buffer, the concentrations of BLf and HSA and pefloxacin mesylate were 1.00×10 −5 mol/L, 1.00×10 −5 mol/L and 5.0×10 −4 mol/L, respectively. To a 1.0 cm quartz cell, BLf and HSA solutions were added to make up 2.5 ml respectively and the range of the drug solution was gradually titrateded into the cell using micro-injector. The accumulated volume was smaller than 200 µl. Under the apparatus condition of both entrance slit and exit slit width being 5 nm, and scanning speed of 240 nm/min, fluorescence quenching spectra and synchronous fluorescence spectra were obtained. Fluorescence quenching spectra were obtained at excitation and emission wavelengths of 295 nm and 300~550 nm for HSA, 290 nm and 295~550 nm for BLf respectively. For absorption spectra (UV) experiments, samples of pefloxacin mesylate were brought to 1.0 cm cuvette versus a blank of buffer. The absorbance was read and spectral scanning curves were made. RESULTS AND DISCUSSION 1 Fluorescence spectra 2 Fig. 2 2 shows the fluorescence spectra of pefloxacin mesylate with BLf and HSA. The fluorescence emission lines of pefloxacin mesylate-BLf (1:1) mixture system and pefloxacin mesylate-HSA (1:1) mixture system can also be observed in Fig. 2 2 . It is well known that the BLf has strong fluorescence at λ ex / λ em =290/333 nm and HSA at λ ex / λ em =295/340 nm. When the solution conditions agree with our studies, Fig. 2 2 shows the maximum strong fluorescence at 418 nm and 410 nm for pefloxacin mesylate respectively. In addition, the BLf and HSA fluorescence spectra are somewhat quenched, indicating interaction has occurred and the energy has been transferred. Binding constant and binding sites 2 For experiments carried out at large molar protein/dye ratios, it was assumed that only strong sites were active in binding dye. For simplicity, these strong binding sites were also assumed to be identical and to act independently. If these assumptions are valid, the site-binding model could be constructed, and the binding equation described by Scatchard is given by (Fletcher et al., 1970 1970 ; Yang et al., 1997 1997 ): $$\nu = nK[D]/(1 + K[D]).$$ Here ν is the average number of dye molecules bound per protein molecule, n is the number of (strong) binding sites, K is the intrinsic (microscopic) binding (association) constant, and [ D ] is the concentration of free (unbound) dye. The linear form equation is as follows: $$\nu /[D] = - K\nu + Kn.$$ Eq.( 2 2 ) is the usual form of Scatchard equation (Fletcher et al., 1970 1970 ; Yang et al., 1997 1997 ). In the case of fluorescence only caused by a protein at the selected wavelength, the relationship between the concentration of protein and the fluorescence intensity can be described by $$F_0 /F = [P_{\text{t}} ]/[P].$$ According to the definition of n , another equation is also known, $$\nu = ([D_{\text{t}} ] - [D])/[P_{\text{t}} ] = n([P_{\text{t}} ] - [P])/[P_{\text{t}} ] = n(F_0 - F)/F_0,$$ where, [ P t ] is the total protein concentration, [ D t ] is the final dye concentration, F 0 and F are, respectively, the fluorescence intensity in the absence of a quencher and in its presence at [ D ] concentration. The following equation would be obtained by combining Eq.( 1 1 ) with Eq.( 4 4 ): $$F_0 /F = K[D_{\text{t}} ]F_0 /(F_0 - F) - nK[P_{\text{t}} ].$$ It indicates that the binding constant K and binding sites n can be obtained at the same time using least-squares algorithm for data-fitting according to Eq.( 5 5 ). The fluorescence quenching spectra of BLf and HSA in Tris-HCl buffer with increasing pefloxacin mesylate concentration and fixed BLf and HSA concentrations (both are 1.00×10 −5 mol/L) are shown in Fig. 3 3 . It is obvious that the concentration of pefloxacin mesylate gradually increases with the titration, the fluorescence intensities of BLf and HSA decrease regularly, at the same time, the fluorescence emission peak of pefloxacin mesylate is gradually enhanced. The well-defined isobestic points are observed at 382 nm for BLf and 368 nm for HSA, which are the direct evidences for drug-protein complex formation. After the fluorescence quenching on BLf at 333 nm and HSA at 340 nm were measured, the linear fit of fluorescence intensity changes of BLf-PFLX system and HSA-PFLX system were assessed by Eq.( 5 5 ). Fig. 4 4 shows the fitting curves and Table 1 1 shows the fitting results. Compound Binding constant, K (L/mol) Binding site, n Correlation coefficient, γ BLf 1.06×10 5 1.85 0.9977 HSA 7.04×10 4 1.13 0.9972 It is indicated that there are strong binding forces between pefloxacin mesylate and BLf as well as pefloxacin mesylate and HSA, and that about two binding sites would be formed for BLf which was the same number as that for iron, but it is uncertain that the binding sites for lactoferrin are equal to those for iron and the two binding sites should have the same binding constant according to the Scatchard equation, and that about one binding site for HSA. Scatchard plot obtained in the present work were both one straight line, which can validate the rationality for the binding model. Binding distance between the drug and the amino acid residues of BLf and HSA 2 According to Förster non-radioactive energy transfer theory (Yang and Gao, 2002 2002 ; Horrocks and Collier 1981 1981 ), the energy transfer effect is related not only to the distance between the acceptor and donor ( r ), but also to the critical energy transfer distance ( R 0 ) (Horrocks and Collier, 1981 1981 ; Ma et al., 1999 1999 ), $$E = R_0^6 /(R_0^6 + r^6 ).$$ Here R 0 is the critical distance when the transfer efficiency is 50%. $$R_0^6 = 8.8 \times 10^{ - 25} K^2 N^{ - 4} \Phi J,$$ where K 2 is the spatial orientation factor of the dipole, N is the refractive index of the medium, Φ is the fluorescence quantum yield of the donor, J is the overlap integral of the fluorescence emission spectrum of the donor and the absorption spectrum of the acceptor (Jiang et al., 2002 2002 ; Shaklai et al., 1977 1977 ). Therefore, $$J{\text{ = }}{{\int_0^\infty {F(\lambda )\varepsilon (\lambda )} \lambda ^4 {\text{d}}\lambda } \mathord{\left/{\vphantom {{\int_0^\infty {F(\lambda )\varepsilon (\lambda )} \lambda ^4 {\text{d}}\lambda } {\int_0^\infty {F(\lambda )} {\text{d}}\lambda }}} \right.\kern-\nulldelimiterspace} {\int_0^\infty {F(\lambda )} {\text{d}}\lambda }}.$$ Here F ( λ ) is the fluorescence intensity of the fluorescent donor at wavelength λ , and ε ( λ ) is the molar absorptivity of the acceptor at wavelength λ , so the energy transfer efficiency is: $$E = 1 - F/F_0 .$$ The overlap of the absorption spectra of pefloxacin mesylate and the fluorescence emission spectra of BLf and HSA are shown in Fig. 5 5 . The overlap integral J can be evaluated by integrating the spectra in Fig. 5 5 . In this paper, J is given by the following Eq.( 10 10 ) and was calculated to be 3.97×10 −15 (cm 3 ·dm 3 )/mol for BLf and 6.15×10 −15 (cm 3 ·dm 3 )/mol for HSA, $$J = \sum {F(\lambda )\varepsilon (\lambda )\lambda ^4 \Delta \lambda /\sum {F(\lambda )\Delta \lambda }. }$$ Under these experimental conditions, the distance corresponding to 50% energy transfer from BLf and HSA to pefloxacin mesylate can be estimated to be R 0 =2.11 nm and R 0 =2.27 nm from Eq.( 7 7 ) using K 2 =2/3, N =1.36, Φ =0.13 (Horrocks and Collier, 1981 1981 ). Moreover, the energy transfer effects are E =0.299 for BLf and E =0.209 for HSA from Eq.( 6 6 ) and the binding distances between pefloxacin mesylate and amino acid residues in BLf and HSA are r =2.82 nm and r =2.83 nm respectively. Effect of the drug on the conformations of BLf and HSA 2 The conformational changes of BLf and HSA were evaluated by the measurement of the synchronous fluorescence intensity of protein amino acid residues before and after the addition of pefloxacin mesylate. Fluorescence measurements give information on the molecular environment in the vicinity of the fluorophore functional groups. In the synchronous spectra, the sensitivity associated with fluorescence is maintained while offering several advantages: spectral simplification, spectral bandwidth reduction and avoiding different perturbing effects. In this work, synchronous fluorescence spectroscopy was used to study the synchronous fluorescence characteristics of BLf and HSA at different scanning interval Δ λ (Δ λ = λ emission − λ excitation ). When Δ λ =15 nm, the spectrum characteristic of the protein tyrosine residues was observed, and when Δ λ =60 nm, the spectrum characteristic of protein tryptophan residues was observed (Ma et al., 1999 1999 ). The authors suggested a useful method to study the environment of amino acid residues is to measure the possible shift in the wavelength emission maximum λ max (Yuan et al., 1998 1998 ; Chen et al., 1990 1990 ). The shift in the position of emission maximum corresponds to the changes in the polarity around the chromophore molecule. Thus, the conformation changes of BLf and HSA can be evaluated by the measurement of λ max . With the concentration of proteins being unchanged, and the concentration of pefloxacin mesylate increasing by titration, the synchronous spectroscopy were scanned at Δ λ =15 nm, Δ λ =60 nm (Figs. 6 6 and 7 7 ). [BLf]: 1×10 −5 mol/L; [PFLX]: x ×10 −5 mol/L, from a to e: x =0, 0.8, 1.6, 2.4, 3.4 [HSA]: 1×10 −5 mol/L; [PFLX]: x ×10 −5 mol/L, from a to i: x =0, 0.4, 0.8, 1.2, 1.6, 2.0, 2.4, 2.8, 3.2 The effect of pefloxacin mesylate on the tyrosine and tryptophan residues fluorescence intensities of BLf and HSA indicates that the main contribution to the fluorescence intensities of BLf and HSA are tryptophan residues. Figs. 6 6 and 7 7 also show the effect of pefloxacin mesylate on the emission maximum with the progression of titration. A little stronger blue-shift of tryptophan fluorescence upon addition of drug was observed, and the emission maximum of tyrosine kept the position. This shift indicates that tryptophan residues were placed in a more hydrophobic environment and less exposed to the solvent. This may be due to the fact that the insertion of pefloxacin mesylate rearranged the tryptophan microenvironment. It also suggested that the environment of tryptophan residues in pure protein solution is relatively polar. Binding of the pefloxacin mesylate changes the environments to apolar ones. The shift in polarity brought about conformational changes by the interaction between protein and the ligand molecule. CONCLUSION 1 The binding interactions of pefloxacin mesylate with BLf and HSA in dilute aqueous solution were studied using fluorescence spectra and absorbance spectra. The results showed that the binding constant ( K ), binding sites ( n ) and the binding distances ( r ) are K =1.06×10 5 L/mol, n =1.85, r =2.82 nm for BLf and K =7.04×10 4 L/mol, n =1.13, r =2.83 nm for HSA, respectively. The effect of pefloxacin mesylate on the conformations of BLf and HSA was also analyzed, with the result indicating that pefloxacin mesylate can affect the conformations of BLf and HSA to some degree. References A Aguila , JH Brock . Lactoferrin: antimicrobial and diagnostic properties . 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