Development of Pasteuria penetrans in Meloidogyne javanica females as affected by constantly high vs fluctuating temperature in an in-vivo system

Development of Pasteuria penetrans in Meloidogyne javanica females as affected by constantly high vs fluctuating temperature in an in-vivo system first-author †E-mail:d_darban@hotmail.com †E-mail:d_darban@hotmail.com d_darban@hotmail.com Department of Agriculture, School of Agriculture, Policy and Development, The University of Reading, UK Department of Agriculture, School of Agriculture, Policy and Development, The University of Reading, UK Growth room and glasshouse experiment was conducted to investigate the effect of constant and fluctuating temperatures on the development of Pasteuria penetrans a hyperparasite of root-knot nematodes. Tomato plants ( Lycopersicon esculentum Mill) were inoculated with Meloidogyne javanica second-stage juveniles attached with endospores of P. penetrans and were grown in growth room at 26–29 °C and in glasshouse at 20–32 °C. The tomato plants were sampled from the growth room after 600 degree-days based on 17 °C/d, accumulating each day above a base temperature of 10 °C and from the glasshouse after 36 calendar days. Temperature affected the development of P. penetrans directly. The rate of development at constant temperature in growth room was faster than that in the glasshouse at fluctuating temperatures. Pasteuria penetrans Meloidogyne javanica Development and tomato plants INTRODUCTION 1 Pasteuria penetrans is an obligate, endospore-forming bacterial parasite of Meloidogyne spp. and has shown potential as a biological control agent against root-knot nematodes (Chen et al., 1996 1996 ; Dickson et al., 1994 1994 ) in pot experiments (Channer and Gowen, 1988 1988 ) and micro-plot experiments (Daudi et al., 1990 1990 ; Trivino and Gowen, 1996 1996 ). There are practical and technical problems associated with mass production of P. penetrans inoculum. In-vivo systems, although proven (Stirling and Wachtel, 1980 1980 ), are unlikely to be adopted for large-scale treatments in most farming systems. If greater use is to be made of in-vivo production techniques, refinements are needed which can only be developed with improvements based on a better understanding of the biological factors involved. The durations and temperatures when mature endospores are first seen in female root-knot nematodes have been recorded (Stirling, 1981 1981 ). Furthermore, Davies et al.( 1988 1988 ) and Giannakou et al.( 1997 1997 ) showed that temperature fluctuations in the glasshouse might retard the development of the parasite. The objective of this work was to determine the effect of different temperature schemes on the development of P. penetrans and number of endospores on a per nematode basis at constant temperatures known to be suitable for P. penetrans and in a fluctuating temperature regime such as might occur in a glasshouse production system. MATERIALS AND METHODS 1 Pasteuria penetrans , originally collected from South Africa but cultured for over 10 years on root-knot nematode hosts at the University of Reading, was established on a population of M. javanica from Pakistan. A spore suspension was prepared by squashing with fine forceps infected females in 0.5 ml water in a small Eppendorf tube and homogenising for 30 s. To the suspension was added 11000 freshly hatched M. javanica J2s which resulted in a spore concentration of 1.22×10 4 spores/ml. After agitation for 2–3 min this was divided between four Petri dishes, which were placed in an incubator at 28 °C to enable a large surface air interface to prolong nematode activity. Spore attachment was monitored hourly until 90% of juveniles were encumbered with 6–10 spores. Then suspensions were combined in one beaker and poured through a 20 μm sieve to separate nematodes from remaining spores in suspension. Eight hundred spore-encumbered J2s were inoculated on 6-week old tomato plants ( Lycopersicon esculentum Mill) cv. Tiny Tim, a dwarf, determinate variety, growing in a loam-based proprietary compost (John Innes No. 2) in 500 cm 3 plastic pots into which they had been transplanted 1 week earlier. For 48 h after inoculation, all plants were kept in the growth room at 26–29 °C to ensure good invasion of nematodes. Then half of the plants were transferred to a glasshouse at 20–32 °C. Each treatment was replicated five times using a randomized block design. All plants were usually watered daily sufficiently to prevent wilting. The daily temperature was recorded by setting electronic temperature loggers (Tiny Talk-II ISO EN 9002) and a probe of each was inserted in soil near to the pots. After three weeks, all pots in the glasshouse were put in horticultural planter bags [92 cm×28 cm×27 cm] containing loam-based compost. The plants from the growth room and glasshouse were sampled after 600 degree-days (equivalent to 36 d) based on 17 °C/d, accumulating each day above a base temperature of 10 °C. After harvesting, all roots were placed in a freezer for 5 d. Upon thawing, the roots readily disintegrated when probed with needles and female root-knot nematodes were collected from the separated root tissue. Fifty endospore-filled females were then individual squashed with fine forceps in a small Eppendorf tube containing 0.5 ml of water and homogenised for 30 s. The suspensions in the Eppendorfs were then rinsed into small glass bottles with 0.5 ml of water. Three aliquots per sample were taken to count the numbers of developing stages (micro colonies, fragmented micro colonies, doublets) and mature endospores of P. penetrans at ×400 magnification using a haemocytometer. RESULTS 1 The temperature had great effect on the development of Pasteuria penetrans , a hyperparasite of root-knot nematodes. The rate of development was found faster and began earlier in the growth room at constantly higher temperature than in the glasshouse at fluctuating temperatures. There was a highly significant ( P < 0.001) difference in the number of micro colonies and was almost three times higher in the glasshouse treatment than in the growth room treatment (Fig. 1 1 ). At fluctuating temperatures the parasite development was not only slower but was more prolonged than that at the constant temperature. There was a highly significant ( P < 0.001) difference in the number of mature endospores per female (Fig. 2 2 ). The highest number of endospores was produced in the growth room. There was a greater difference between glasshouse and growth room in the development of P. penetrans . At 36 calendar days (600 degree-days) in the glasshouse treatment, the development of P. penetrans was slower than that in the growth room treatment, which was clearly indicated by the fewer doublets and mature endospores. However, more micro colonies and fragmented micro colonies were found in the glasshouse treatment than in the growth room treatment (Fig. 1 1 ). DISCUSSION 1 This research was done to determine the conditions necessary to maximize the production of endospores of Pasteuria penetrans on a per nematode basis. It was hypothesised that more spores would be produced in nematodes at constantly higher temperature than at fluctuating temperature. Hatz and Dickson ( 1992 1992 ) reported that with increasing temperature the development of P. penetrans and the formation of mature endospores began earlier. After 600 degree-days in the growth room, it has been observed that continuous higher temperature promoted the faster development of nematodes which indirectly promoted the development of hyper parasite P. penetrans , as clearly indicated by the higher number of endospores produced at constantly higher temperature in the growth room (Fig. 2 2 ). Plants in the glasshouse at fluctuating lower temperature were healthier than those in the growth room and produced bigger root systems. It could explain that healthier and bigger root systems of the glasshouse plants provided more space and less food competition than those in the growth room where the slower the nematode development indirectly affected the development of P. penetrans . Giannakou et al.( 1999 1999 ) found that a bigger root system provides better conditions for the development of female nematodes which in turn provides a better medium for the development of the parasite. Studies by Hatz and Dickson ( 1992 1992 ) also showed that there is coincident development by host (nematode) and hyperparasite ( Pasteuria ). These results show that in-vivo systems of production are feasible. In field-grow production there will be less diurnal temperature fluctuation and more natural conditions than in a glasshouse or the growth room. References AG Channer , SR Gowen . 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