Olig2<sup>+</sup> single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model

Deqing PENG; Ruijie LU; Leyao LÜ; Qing YAO; Kaichuang YANG; Yunfeng XU; Xiaoming FENG; Ruolang PAN; Yuyuan MA

doi:10.1631/jzus.B2300790

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Olig2⁺ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model

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Correspondence | Updated：2024-09-26

Olig2⁺ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model
Olig2⁺ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model
Olig2⁺单克隆颅骨间充质干细胞在双环己酮草酰二腙（CPZ）诱导的小鼠脱髓鞘模型中促再生作用研究
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) Pages: 1-7(2024)
Affiliations：

1.Center for Rehabilitation Medicine, Department of Neurosurgery, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou 310014, China
2.Second Clinical Medical College, Wenzhou Medical University, Wenzhou 325035, China
3.School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China
4.Department of Neurosurgery, the First Hospital of Jiaxing (Affiliated Hospital of Jiaxing University), Jiaxing 314001, China
5.Key Laboratory of Cell-Based Drug and Applied Technology Development in Zhejiang Province, Hangzhou 311122, China
Funds：
DOI：10.1631/jzus.B2300790
CLC：
CSTR：
Published Online： 26 September 2024 ，

Received： 30 October 2023 ，

Revised： 24 April 2024 ，
Accepted：

彭德清,卢睿杰,吕乐瑶等.Olig2,⁺单克隆颅骨间充质干细胞在双环己酮草酰二腙（CPZ）诱导的小鼠脱髓鞘模型中促再生作用研究[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）,

Deqing PENG, Ruijie LU, Leyao LÜ, et al. Olig2⁺ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model. [J/OL]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2024,1-7.
彭德清,卢睿杰,吕乐瑶等.Olig2,⁺单克隆颅骨间充质干细胞在双环己酮草酰二腙（CPZ）诱导的小鼠脱髓鞘模型中促再生作用研究[J].浙江大学学报（英文版）（B辑：生物医学和生物技术）, DOI：10.1631/jzus.B2300790.

Deqing PENG, Ruijie LU, Leyao LÜ, et al. Olig2⁺ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model. [J/OL]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2024,1-7. DOI： 10.1631/jzus.B2300790.

摘要

多发性硬化症是中枢神经系统的主要脱髓鞘疾病，目前尚无有效治疗方法。本研究探讨了颅骨间充质干细胞（cBMMSC）和Olig2

单克隆来源cBMMSC（sc-cBMMSC）在双环己酮草酰二腙（CPZ）诱导的中枢神经系统脱髓鞘小鼠模型中的作用。通过尾静脉输注cBMMSC和Olig2

sc-cBMMSC，剂量为1×10

个细胞每100微升磷酸盐缓冲液（PBS），每周一次，给药两周。治疗完成一周后，对每组小鼠进行相关指标评估。结果表明，cBMMSC移植可显著提高CPZ诱导的脱髓鞘小鼠在转棒、悬挂和水迷宫试验中的能力；抑制小鼠外周血和中枢神经系统中TNF-α、IL-1β和IL-6等炎症因子的水平；此外，小鼠病理结构也得到改善，具体表现在髓鞘得到显著恢复，以及Olig2和Syn表达增加。值得注意的是，与cBMMSC相比，Olig2

sc-cBMMSC更为显著地改善了CPZ动物的行为学、髓鞘修复和神经细胞再生。这些发现表明，颅骨髓来源的干细胞在脱髓鞘中具有修复作用，这可能归因于其对少突胶质细胞的免疫调节和保护。Olig2

sc-cBMMSC移植效果更为显著，进一步证实了间充质干细胞的异质性应被考虑和利用以提高对特定疾病的治疗效果。

Abstract

关键词

颅骨骨髓单克隆间充质干细胞少突胶质细胞脱髓鞘

Keywords

references

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Olig2+ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model

Olig2+ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model

DOI：10.1631/jzus.B2300790

摘要

Abstract

关键词

Keywords

references

Olig2⁺ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model

Olig2⁺ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model