The protective effect of folic acid on oxidative stress induced by lead acetate in PC12 cells

The protective effect of folic acid on oxidative stress induced by lead acetate in PC12 cells Ning Li1 first-author Mingming Li 1 Xianqing Huang 1 Lianjun Song 1 Mingwu Qiao 1 Tianlin Wang 1 Tiange Li 1 Xiuhong Zhu 2 Can Cui 1 College of Food Science and Technology, Henan Agricultural University,Zhengzhou,China,450002 College of Food Science and Technology, Henan Agricultural University,Zhengzhou,China,450002 College of Forestry, Henan Agricultural University,Zhengzhou,China,450002 College of Forestry, Henan Agricultural University,Zhengzhou,China,450002 Folic acid (FA) plays an important role in cell metabolism. In this study, we primarily looked at the protective effect of FA on PC12 cytotoxicity induced by lead acetate. PC12 cells were exposed to lead-free medium and 10 & #x3BC;mol/L lead-acetate culture solution for 24h. Medium with FA proportions of 0mg/L, 0.5mg/L, 5mg/L, and 50mg/L continued to function. After 24h, 48h, and 72h, we collected PC12 cells, and detected cell proliferation or inhibition with the MTT method. We extracted cell protein and detected the activities of SOD, MDA, and GSH-PX in each group. The immunofluorescence method was used to study the expression of caspase-3 protein. Changes of A & #x3B2; 40 and A & #x3B2; 42 in the cell-culture fluid were detected with ELISA. The results showed that lead exposure inhibited cell growth ( P < 0.05), increased intracellular MDA content, SOD activity, and caspase-3 protein expression ( P < 0.05), and decreased GSH-PX activity and A & #x3B2; 40 protein expression ( P < 0.05). FA intervention can increase cell proliferation, enhance the activity of SOD and GSH-PX ( P < 0.05), and reduce cell MDA content and caspase-3 protein expression ( P < 0.05). There was no statistically significant difference between A & #x3B2; 40 and A & #x3B2; 42 content between the groups ( P > 0.05). The study found that FA could be a highly effective approach against lead-associated PC12 cell toxicity. Lead Folic acid PC12 cells Oxidative stress Apoptosis 10.1631/jzus.B2100938 2021-11-15 2022-03-04 2022-06-07 2022-06-07